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Anti-S6K1 (phospho T229)抗体(ab59208)

Reviews (2)Q&A (3)References (32)
Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho T229) antibody (ab59208)
  • Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)
  • Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)

Key features and details

  • Rabbit polyclonal to S6K1 (phospho T229)
  • Suitable for: WB, IHC-P, ELISA
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-S6K1 (phospho T229)抗体
    参阅全部 S6K1 一抗

  • 描述

    兔多克隆抗体to S6K1 (phospho T229)

  • 宿主

    Rabbit

  • 特异性

    Detects endogenous levels of S6K1 only when phosphorylated at threonine 229 for human and rat, threonine 252 for mouse. This antibody was specifically designed for detection of S6K1 and binds to the protein kinase domain of isoforms alpha I and alpha II. We have some feedback to indicate that is may not be able to detect S6K2, but this has not been confirmed.

  • 经测试应用

    适用于: WB, IHC-P, ELISAmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human S6K1 aa 150-250 (phospho T229). Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 229 (THTPFC)
    Database link: P23443

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab59208于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
说明
WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

靶标

  • 功能

    Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.

  • 组织特异性

    Widely expressed.

  • 序列相似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.

  • 结构域

    The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
    The TOS (TOR signaling) motif is essential for activation by mTORC1.

  • 翻译后修饰

    Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.

  • 细胞定位

    Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 70 kDa ribosomal protein S6 kinase 1 antibody
    • KS6B1_HUMAN antibody
    • p70 alpha antibody
    • P70 beta 1 antibody
    • p70 ribosomal S6 kinase alpha antibody
    • p70 ribosomal S6 kinase beta 1 antibody
    • p70 S6 kinase alpha antibody
    • P70 S6 Kinase antibody
    • p70 S6 kinase, alpha 1 antibody
    • p70 S6 kinase, alpha 2 antibody
    • p70 S6K antibody
    • p70 S6K-alpha antibody
    • p70 S6KA antibody
    • p70(S6K) alpha antibody
    • p70(S6K)-alpha antibody
    • p70-alpha antibody
    • p70-S6K 1 antibody
    • p70-S6K antibody
    • P70S6K antibody
    • P70S6K1 antibody
    • p70S6Kb antibody
    • PS6K antibody
    • Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase beta 1 antibody
    • Ribosomal protein S6 kinase beta-1 antibody
    • Ribosomal protein S6 kinase I antibody
    • RPS6KB1 antibody
    • S6K antibody
    • S6K-beta-1 antibody
    • S6K1 antibody
    • Serine/threonine kinase 14 alpha antibody
    • Serine/threonine-protein kinase 14A antibody
    • STK14A antibody
    see all

图片

  • Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)
    Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)
    All lanes : Anti-S6K1 (phospho T229) antibody (ab59208) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunizing phosphopeptide

    Predicted band size: 53 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho T229) antibody (ab59208)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho T229) antibody (ab59208)
    Immunohistochemical analysis of paraffin embedded human brain tissue using ab59208 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
  • Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)
    Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)This image is a courtesy of Anonymous Abreview
    Anti-S6K1 (phospho T229) antibody (ab59208) at 1/500 dilution + Lysates prepared from human HT1080 cell line at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes

    See Abreview

  • Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)
    Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)This image is a courtesy of Anonymous Abreview
    Anti-S6K1 (phospho T229) antibody (ab59208) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes

    See Abreview

文献 (32)

发表研究结果有使用 ab59208？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab59208 被引用在 32 文献中.

  • Jin Z & Jin Y Raw Lacquer Extract from Toxicodendron vernicifluum in Combination with ONC201 Enhances the Inhibitory Effects on Colorectal Cancer Cell Activity. Turk J Gastroenterol 34:211-220 (2023). PubMed: 36511604
  • Jia W  et al. Negative regulation of HBG1/2 expression through S6K by long noncoding RNA NR_120526. Transl Pediatr 12:907-917 (2023). PubMed: 37305725
  • Pan X  et al. Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells. Mol Med Rep 28:N/A (2023). PubMed: 37594055
  • Lu J  et al. Activation of acetyl-CoA synthetase 2 mediates kidney injury in diabetic nephropathy. JCI Insight 8:N/A (2023). PubMed: 37870960
  • Wei L  et al. CMTM6 knockdown prevents glioma progression by inactivating the mTOR pathway. Ann Transl Med 10:181 (2022). PubMed: 35280358
View all Publications for this product

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-P70 S6 Kinase alpha (phospho T229) antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 cell line)
Loading amount
10 µg
Specification
HT1080 cell line
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 14 2010

Western blot abreview for Anti-P70 S6 Kinase alpha (phospho T229) antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cell line)
Loading amount
10 µg
Specification
NIH 3T3 cell line
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 18 2009

Question

Hi!

Thank you for your answer. As the antibody is stated in the product info to be directed towards pS6K2, and it is now said that it was designed for pS6K1. We want a total refund of the antibody and delivery costs. All the work that has been done with this antibody in our lab, all the samples that were used, I guess cannot be replaced by money, unfortunately.

Please sent the credit invoice to:

#####

Read More
Answer

Thank you for your message.

I would be pleased to provide a credit note for you as requested. In order to do so, I would appreciate if you could please provide the original order number and date of purchase.

Thank you for your time. I look forward to hearing from you.

Read More

Question

Inquiry: * 20µg * BSA for blocking and antibody dilution * ECL, CCD camera for pS6K2 * 1:750 Over Night at 4degrees C * 1:1500 1h at RT * TBS + 0.1% tween The antibody was tested with both WB and IHC after control siRNA, S6K1siRNA, S6K2siRNA, and both S6k1 and2 SiRNA. The picture shows that the antibody detects S6K1, since the bands dissappear after S6K1siRNA treatment for 72h. Previously, in other lysates I have detected the S6K2 total protein to dissappear after S6K2siRNA treatment in exactly the same conditions as this experiment shown in the picture. After very much work with an antibody that do not detect what it should, it feels terrible, and I wonder if you have an other antibody that really detects the pS6K2, and not pS6K1. Also I would lika a refund on this antibody. Regards

Read More
Answer

Thank you for taking the time to contact us and provide this information. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab59208 is tested and covered by our 6 month guarantee for use in WB and human, rat and mouse samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.


Reviewing this case, I can confirm for you that the amino acid range of this immunogen for this antibody is #####. This antibody binds to the protein kinase domain of isoforms alpha I and alpha II. Also, this antibody was designed specifically against S6K1, which seems to work well in these experiments.

I fully understand your concerns, and it is regrettable the antibody has not worked as you anticipated. Since detection of S6K2 seems to be the issue, it may be that the antibody may not be able to bind to S6K2 which would be expected if the antibody is designed for detection of S6K1.

I have added this information to the online datasheet in order to ensure this is clear for customers.

I hope this will help to explain the results. Please do not hesitate to contact me again if you have any further concerns or questions.

Read More

Question

Wrong band size in Western blot with human cancer cell lines.

Read More
Answer

Thanks for your phone call today and for letting us know about the trouble with ab59208 in Western blot. As we discussed, I am sending a vial of ab48394 free of charge on the order ***. This product is in stock and should arrive tomorrow. I hope this replacement vial will work well for you. Please keep me updated, and let me know if there is anything else that we can do for you.

Read More

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