Anti-S6K1 (phospho T229)抗体(ab5231)
Key features and details
- Rabbit polyclonal to S6K1 (phospho T229)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-S6K1 (phospho T229)抗体
参阅全部 S6K1 一抗 -
描述
兔多克隆抗体to S6K1 (phospho T229) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human S6K1 (phospho T229). The sequence is conserved in rat.
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阳性对照
- Hek293 cells +/- EGF; truncated p70S6K protein, an N-terminus His-tagged fusion protein corresponding to amino acids 1-421 of human p70S6K containing a T412E mutation to become constitutively active.
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常规说明
70 kDa ribosomal protein S6 kinase (p70S6K or p70S6K beta 1) is a member of a serine/threonine kinase family that phosphorylates the 40S ribosomal protein S6, thereby modulating the translation of ribosomal proteins and translation elongation factors. p70S6K is activated in response to the phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) pathways, and is required for progression through the cell cycle and for cell growth. Activation of p70S6K is regulated by phosphorylation of seven different residues distributed throughout the protein, with the critical sites being threonines 229, 389, 421 and serine 424. Threonine 229 is located within the activation loop.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p70S6K. The final product is generated by affinity chromatography using a p70S6K derived peptide that is phosphorylated at threonine 229. -
Primary antibody说明
70 kDa ribosomal protein S6 kinase (p70S6K or p70S6K beta 1) is a member of a serine/threonine kinase family that phosphorylates the 40S ribosomal protein S6, thereby modulating the translation of ribosomal proteins and translation elongation factors. p70S6K is activated in response to the phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) pathways, and is required for progression through the cell cycle and for cell growth. Activation of p70S6K is regulated by phosphorylation of seven different residues distributed throughout the protein, with the critical sites being threonines 229, 389, 421 and serine 424. Threonine 229 is located within the activation loop. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 70 kDa.
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| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 70 kDa. |
靶标
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功能
Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
结构域
The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1. -
翻译后修饰
Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. -
细胞定位
Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6198 Human
- Omim: 608938 Human
- SwissProt: P23443 Human
- Unigene: 463642 Human
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别名
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
see all
图片
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Western blot - Anti-S6K1 (phospho T229) antibody (ab5231)Extracts prepared from Hek293 cells (1-4) or activated p70 S6 kinase protein (5, 6) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either untreated (1-5), or treated with lambda (ë) phosphatase (6), blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with 0.50
µ g/mL ab5231 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: nopeptide (1, 5, 6), the non-phosphorylated peptide corresponding to the phosphopeptide (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4).After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected using the Tropix WesternStar method.The data show that only the phosphopeptide corresponding to p70S6K [pT229] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the si
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab5231 被引用在 15 文献中.
- Park J et al. A novel small molecule, CU05-1189, targeting the pleckstrin homology domain of PDK1 suppresses VEGF-mediated angiogenesis and tumor growth by blocking the Akt signaling pathway. Front Pharmacol 14:1275749 (2023). PubMed: 38035024
- Jiang Q et al. S6K1-mediated phosphorylation of PDK1 impairs AKT kinase activity and oncogenic functions. Nat Commun 13:1548 (2022). PubMed: 35318320
- Zhou X et al. PI3K inhibition sensitizes EGFR wild-type NSCLC cell lines to erlotinib chemotherapy. Exp Ther Med 21:9 (2021). PubMed: 33235618
- Chen S et al. Salidroside protects SH-SY5Y from pathogenic a-synuclein by promoting cell autophagy via mediation of mTOR/p70S6K signaling. Mol Med Rep 20:529-538 (2019). PubMed: 31180515
- Arif A et al. Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate Selection. Mol Cell 73:446-457.e6 (2019). PubMed: 30612880