Anti-S6K1 (phospho S424)抗体(ab131436)
Key features and details
- Rabbit polyclonal to S6K1 (phospho S424)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-S6K1 (phospho S424)抗体
参阅全部 S6K1 一抗 -
描述
兔多克隆抗体to S6K1 (phospho S424) -
宿主
Rabbit -
特异性
ab131436 detects endogenous levels of S6K only when phosphorylated at serine 424. -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human S6K1 (phospho S424) conjugated to keyhole limpet haemocyanin.
Database link: P23443-2 -
阳性对照
- MCF7 cell extract treated with EGF; HeLa cells; Human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab131436 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131436于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 70, 85 kDa (predicted molecular weight: 56, 59 kDa).
|
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| IHC-P |
1/50 - 1/100.
|
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| ICC/IF |
1/100 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 70, 85 kDa (predicted molecular weight: 56, 59 kDa). |
|
IHC-P
1/50 - 1/100. |
|
ICC/IF
1/100 - 1/200. |
靶标
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功能
Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
结构域
The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1. -
翻译后修饰
Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. -
细胞定位
Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6198 Human
- Omim: 608938 Human
- SwissProt: P23443 Human
- Unigene: 463642 Human
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别名
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
see all
图片
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Western blot - Anti-S6K1 (phospho S424) antibody (ab131436)All lanes : Anti-S6K1 (phospho S424) antibody (ab131436) at 1/500 dilution
Lane 1 : MCF7 cell extract untreated
Lane 2 : MCF7 cell extract treated with EGF
Predicted band size: 56, 59 kDa
Observed band size: 70,85 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho S424) antibody (ab131436)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling S6K (phospho S424) with ab131436 at 1/50 dilution. Right panel was preincubated with blocking peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-S6K1 (phospho S424) antibody (ab131436)Immunofluorescent analysis of methanol-fixed HeLa cells labelling S6K (phospho S424) with ab131436 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab131436 被引用在 18 文献中.
- Du X et al. Evaluation of hepatic AMPK, mTORC1, and autophagy-lysosomal pathway in cows with mild or moderate fatty liver. J Dairy Sci 107:3269-3279 (2024). PubMed: 37977448
- Zhu S et al. DNA methylation and miR-92a-3p-mediated repression of HIP1R promotes pancreatic cancer progression by activating the PI3K/AKT pathway. J Cell Mol Med 27:788-802 (2023). PubMed: 36811277
- Li G et al. Downregulation of NEDD4L by EGFR signaling promotes the development of lung adenocarcinoma. J Transl Med 20:47 (2022). PubMed: 35090513
- Wang Y et al. The synergistic antitumor effect of IL-6 neutralization with NVP-BEZ235 in hepatocellular carcinoma. Cell Death Dis 13:146 (2022). PubMed: 35165269
- Zheng H et al. Dysregulated pseudogene BNIP3P1 inhibited cell proliferation and promoted cell apoptosis in preeclampsia by acting as a competing endogenous RNA for BNIP3. Environ Toxicol 37:971-982 (2022). PubMed: 35187800