重组Anti-S1P1/EDG1抗体[EPR21202] (ab233386)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21202] to S1P1/EDG1
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-S1P1/EDG1抗体[EPR21202]
参阅全部 S1P1/EDG1 一抗 -
描述
兔单克隆抗体[EPR21202] to S1P1/EDG1 -
宿主
Rabbit -
特异性
WB is not recommended for mouse samples.
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经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat and BxPC-3 whole cell lysates, SKHEP1 lysate; IHC-P: Human colon, diffuse large B-cell lymphoma and kidney tissue; Mouse kidney, cerebral cortex and spleen tissue; ICC/IF: SKHEP1 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR21202 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab233386于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Not recommended for mouse. Please do not boil the sample before loading the gel. |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/400.
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说明 |
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WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). Not recommended for mouse. Please do not boil the sample before loading the gel. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/400. |
靶标
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功能
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins. -
组织特异性
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1901 Human
- Entrez Gene: 13609 Mouse
- Omim: 601974 Human
- SwissProt: P21453 Human
- SwissProt: O08530 Mouse
- Unigene: 154210 Human
- Unigene: 982 Mouse
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别名
- CD363 antibody
- CHEDG 1 antibody
- CHEDG1 antibody
see all
图片
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All lanes : Anti-S1P1/EDG1 antibody [EPR21202] (ab233386) at 1/1000 dilution
Lane 1 : Wild-type SKHEP1 lysate
Lane 2 : S1PR1 knock-out SKHEP1 lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
ab233386 was shown to react with S1PR1 in wild-type SKHEP1 cells in Western blot with loss of signal observed in a S1PR1 knockout cell line. Wild-type SKHEP1 and S1PR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab233386 overnight at 4°C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2µg/mL before imaging.
This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies. -
ab233386 was shown to react with S1PR1 in wild-type SKHEP1 cells in immunocytochemistry with loss of signal observed in a S1PR1 knockout cell line. Wild-type and knockout cells were mixed and pelleted at a 1:1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 1x PBS, 0.01% Triton X-100, 5% BSA, 5% NGS. The cells were then incubated with ab233386 at 1:400dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-rabbit secondary antibody to (Alexa Fluor® 555) at 0.5 μg/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (knockout) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and knockout cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880).
This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in endothelial cells of human colon (PMID:25588843) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
All lanes : Anti-S1P1/EDG1 antibody [EPR21202] (ab233386) at 1/1000 dilution
Lanes 1 & 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lanes 2 & 4 : BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Exposure time: 114 secondsBlocking buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in endothelial cells of human kidney (PMID:25588843) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells and tumor cells of human diffuse large B-cell lymphoma (PMID: 22745305) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of mouse kidney (PMID: 25588843) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of mouse spleen(PMID: 25588843) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of mouse cerebral cortex (PMID: 25588843) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab233386 被引用在 3 文献中.
- Liu JX et al. Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2. Front Cell Infect Microbiol 12:1011378 (2022). PubMed: 36339341
- Jia L et al. Triptolide Inhibits the Biological Processes of HUVECs and HepG2 Cells via the Serine Palmitoyltransferase Long Chain Base Subunit 2/Sphingosine-1-Phosphate Signaling Pathway. Dis Markers 2022:9119423 (2022). PubMed: 36438896
- Ye M et al. A Novel Radioimmune 99mTc-Labeled Tracer for Imaging Sphingosine 1-Phosphate Receptor 1 in Tumor Xenografts: An In Vitro and In Vivo Study. Front Immunol 12:660842 (2021). PubMed: 34484174