Anti-S1P1/EDG1抗体(ab77076)
Key features and details
- Rabbit polyclonal to S1P1/EDG1
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-S1P1/EDG1抗体
参阅全部 S1P1/EDG1 一抗 -
描述
兔多克隆抗体to S1P1/EDG1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- mouse thymus tissue lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77076于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
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| IHC-P | (2) |
Use a concentration of 2.5 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa. |
|
IHC-P
Use a concentration of 2.5 µg/ml. |
靶标
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功能
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins. -
组织特异性
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 13609 Mouse
- SwissProt: O08530 Mouse
- Unigene: 982 Mouse
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别名
- CD363 antibody
- CHEDG 1 antibody
- CHEDG1 antibody
see all
图片
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Western blot - Anti-S1P1/EDG1 antibody (ab77076)Lane 1 : Anti-S1P1/EDG1 antibody (ab77076) at 1 µg/ml
Lane 2 : Anti-S1P1/EDG1 antibody (ab77076) at 2 µg/ml
All lanes : mouse thymus tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody (ab77076)Immunofluorescence of S1P1/EDG1 in mouse thymus tissue with ab77076 at 20 μg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody (ab77076)Immunohistochemistry of S1P1/EDG1 in mouse thymus tissue with ab77076 at 5 μg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody (ab77076)Immunohistochemistry of S1P1/EDG1 in mouse thymus tissue with ab77076 at 2.5 μg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab77076 被引用在 6 文献中.
- Yang CH et al. Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain. Cell Rep 30:959-968.e3 (2020). PubMed: 31995766
- Li Y et al. The imbalance in the aortic ceramide/sphingosine-1-phosphate rheostat in ovariectomized rats and the preventive effect of estrogen. Lipids Health Dis 19:95 (2020). PubMed: 32430006
- Chen A et al. Inhibition of miR-155-5p attenuates the valvular damage induced by rheumatic heart disease. Int J Mol Med 45:429-440 (2020). PubMed: 31894293
- Ji Y et al. Potential biomarkers Ang II/AT1R and S1P/S1PR1 predict the prognosis of hepatocellular carcinoma. Oncol Lett 20:208 (2020). PubMed: 32963614
- Guan Z et al. Sphingosine-1-Phosphate Evokes Unique Segment-Specific Vasoconstriction of the Renal Microvasculature. J Am Soc Nephrol N/A:N/A (2014). PubMed: 24578134
- Zhu Q et al. Low-dose cytokine-induced neutral ceramidase secretion from INS-1 cells via exosomes and its anti-apoptotic effect. FEBS J 281:2861-70 (2014). PubMed: 24798654