AB282589

重组Anti-S1P1/EDG1抗体[EPR23535-146] - BSA and Azide free

Anti-S1P1/EDG1 antibody [EPR23535-146] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal S1P1/EDG1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples.

查看别名

CD363, Edg1, Lpb1, S1pr1, Sphingosine 1-phosphate receptor 1, S1P receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, Lysophospholipid receptor B1, Sphingosine 1-phosphate receptor Edg-1, S1P receptor Edg-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR23535-146] - BSA and Azide free (AB282589)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR23535-146] - BSA and Azide free (AB282589)

This data was developed using ab259902, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling S1P1/EDG1 with ab259902 at 1/100 (5.08 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Cytoplasmic staining in rat cerebrum.The section was incubated with ab254360 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR23535-146] - BSA and Azide free (AB282589)

This data was developed using ab259902, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling S1P1/EDG1 with ab259902 at 1/100 (5.08 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Cytoplasmic staining in mouse cerebrum.The section was incubated with ab254360 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

Supplier Data

Immunoprecipitation - Anti-S1P1/EDG1 antibody [EPR23535-146] - BSA and Azide free (AB282589)

This data was developed using ab259902, the same antibody clone in a different buffer formulation.

S1P1/EDG1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab259902 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259902 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 ug

Lane 2 : ab259902 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259902 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5.5 seconds

Sample loaded onto lane 1 is non-boiled as boiling may cause protein aggregates.

All lanes:

Immunoprecipitation - Anti-S1P1/EDG1 antibody [EPR23535-146] (<a href='/products/primary-antibodies/s1p1-edg1-antibody-epr23535-146-ab259902'>ab259902</a>)

Predicted band size: 43 kDa

Observed band size: 42 kDa

false

Lab

Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] - BSA and Azide free (AB282589)

This data was developed using ab259902, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID : 19810093, 14688232).

Low expression : testis (PMID : 9931453).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 48 seconds

All lanes:

Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] (<a href='/products/primary-antibodies/s1p1-edg1-antibody-epr23535-146-ab259902'>ab259902</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate 20 at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 43 kDa

Observed band size: 48 kDa

false

Lab

Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] - BSA and Azide free (AB282589)

This data was developed using ab259902, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Low expression : testis (PMID : 9931453).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 48 seconds

All lanes:

Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] (<a href='/products/primary-antibodies/s1p1-edg1-antibody-epr23535-146-ab259902'>ab259902</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 43 kDa

Observed band size: 42 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-S1P1/EDG1 antibody [EPR23535-146]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23535-146

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat

应用

WB, IHC-P, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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产品详情

ab282589 is the carrier-free version of ab259902.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The S1P1 receptor also known as EDG1 is a G-protein-coupled receptor that plays a significant role in the sphingosine-1-phosphate (S1P) signaling pathway. With a molecular mass of approximately 43 kDa S1P1 is expressed in various tissues such as vascular endothelial cells immune cells and cardiac cells. The protein mediates the effects of sphingosine-1-phosphate a bioactive lipid that participates in various physiological processes.

Biological function summary

S1P1 is involved in the regulation of immune cell trafficking and vascular stability. As a part of a receptor complex it activates intracellular signaling cascades that influence cell migration and vascular maturation. By binding to S1P S1P1 controls the egress of lymphocytes from lymphoid tissues indicating its important role in immune surveillance and response.

Pathways

S1P1 serves as an important component in the S1P signaling pathway and the phosphoinositide 3-kinase (PI3K) pathway. In the S1P pathway it regulates endothelial cell barrier function and vascular maturation. In the PI3K pathway S1P1 influences cell survival and migration which relates it to protein kinases like Akt and other signaling molecules that further modify cellular responses.

S1P1 is implicated in multiple sclerosis (MS) and cancer progression. In MS S1P1 modulation affects lymphocyte trafficking which is the mechanism behind the action of fingolimod (FTY720) a therapeutic agent for the disease. S1P1 also connects to VEGF and other angiogenic factors in cancer where it influences tumor angiogenesis and metastasis. Understanding these connections highlights the potential for targeting S1P1 in therapeutic strategies for these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis. Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury.

See full target information S1pr1

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重组Anti-S1P1/EDG1抗体[EPR23535-146] - BSA and Azide free