Anti-S1P抗体(ab59870)
Key features and details
- Rabbit polyclonal to S1P
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-S1P抗体
参阅全部 S1P 一抗 -
描述
兔多克隆抗体to S1P -
宿主
Rabbit -
特异性
We have a range of domain specific antibodies for this target. For a full list please see all MBTPS1 antibodies. ab59870 recognizes all four forms of MBTPS1 (1052, 746, 607 and 552 amino acids in length). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide based on the catalytic domain of human MBTPS1. Immunizing peptide available on request - please contact a member of staff to ask for availability.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59870于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 118 kDa. 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates.
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| 说明 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 118 kDa. 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates. |
靶标
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相关性
S1P (also known as MBTPS1,SKI1), was first discovered as one of two enzymes that cleave the sterol regulatory binding proteins (SREBPs), in the case of S1P at “site-1,” a hydrophillic loop extending into the lumen of the ER. The MBTPS1 domain structure contains a signal sequence followed by a propeptide domain, a catalytic domain, a transmembrane domain and a cytoplasmic domain. -
细胞定位
Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. -
数据库链接
- Entrez Gene: 8720 Human
- Omim: 603355 Human
- SwissProt: Q14703 Human
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别名
- kexin isozyme 1 antibody
- KIAA0091 antibody
- MBTPS1 antibody
see all
图片
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Western blot - Anti-S1P antibody (ab59870)Anti-S1P antibody (ab59870) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Exposure time: 16 minutes
The 148 kDa zymogen is processed progressively into two membrane-bound 120 and 106 kDa forms in the endoplasmic reticulum, and late into a secreted 98 kDa form. The propeptide is autocatalytically removed through an intramolecular cleavage after Leu-186. Further cleavage generates 14, 10, and 8 kDa intermediates.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab59870 被引用在 2 文献中.
- Chen Y et al. SPK1/S1P axis confers gastrointestinal stromal tumors (GISTs) resistance of imatinib. Gastric Cancer 26:26-43 (2023). PubMed: 35999321
- Tang W et al. Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway-implications for Parkinson's disease. Hum Mol Genet 32:1466-1482 (2023). PubMed: 36519761