重组Anti-S100P抗体[EPR6143] (ab133554)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6143] to S100P
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-S100P抗体[EPR6143]
参阅全部 S100P 一抗 -
描述
兔单克隆抗体[EPR6143] to S100P -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human S100P aa 50 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P25815 -
阳性对照
- WB: SW480 and BxPC-3 cell lysates and human placenta tissue lysate. IHC-P: Human pancreatic adenocarcinoma and placenta tissues. ICC/IF: SW480 cells. IP: SW480 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 3.16 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6143 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133554于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
For unpurified use at 1/200. |
|
IHC-P | (1) |
1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/150 - 1/100. |
ICC/IF |
1/400.
For unpurified use at 1/40. |
|
IP |
1/20.
For unpurified use at 1/80. |
|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). For unpurified use at 1/200. |
IHC-P
1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/150 - 1/100. |
ICC/IF
1/400. For unpurified use at 1/40. |
IP
1/20. For unpurified use at 1/80. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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组织特异性
Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias. -
序列相似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
细胞定位
Nucleus. Cytoplasm. Colocalizes with S100PBP in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6286 Human
- Omim: 600614 Human
- SwissProt: P25815 Human
- Unigene: 2962 Human
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别名
- MIG9 antibody
- Migration inducing gene 9 antibody
- Protein S100-E antibody
see all
图片
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All lanes : Anti-S100P antibody [EPR6143] (ab133554) at 1/200 dilution (unpurified)
Lane 1 : SW480 cell lysate
Lane 2 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling S100P with unpurified ab133554 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of SW480 cells labelling S100P (green) with pruified ab133554 at 1/40. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling S100P with purified ab133554 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic adenocarcinoma tissue labelling S100P with unpurified ab133554 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of BxPC-3 (human Pancreas adenocarcinoma) cells labeling S100P with purified ab133554 at 1/800 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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All lanes : Anti-S100P antibody [EPR6143] (ab133554) at 1/1500 dilution (purified)
Lane 1 : SW480 cell lysate
Lane 2 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-S100P antibody [EPR6143] (ab133554) at 1/1000 dilution (unpurified)
Lane 1 : SW480 cell lysate
Lane 2 : Human placenta cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa -
Immunocytochemistry/Immunofluorescence analysis of SW480 cells labelling S100P (green) with pruified ab133554 at 1/400. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
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ab133554 (unpurified) at 1/20 immunoprecipitating S100P in SW480 cells. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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ab133554 (purified) at 1/80 immunoprecipitating S100P in SW480 cells. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab133554 被引用在 13 文献中.
- Wu X et al. Multiomic landscape of immune pathogenesis in Kimura's disease. iScience 26:106559 (2023). PubMed: 37123228
- Song G et al. Single-cell transcriptomic analysis suggests two molecularly subtypes of intrahepatic cholangiocarcinoma. Nat Commun 13:1642 (2022). PubMed: 35347134
- Lin M et al. S100P contributes to promoter demethylation and transcriptional activation of SLC2A5 to promote metastasis in colorectal cancer. Br J Cancer 125:734-747 (2021). PubMed: 34188196
- Zhang D et al. Down-regulation of S100P induces apoptosis in endometrial epithelial cell during GnRH antagonist protocol. Reprod Biol Endocrinol 19:99 (2021). PubMed: 34215278
- Lu C et al. Knockdown of ferritin heavy chain (FTH) inhibits the migration of prostate cancer through reducing S100A4, S100A2, and S100P expression. Transl Cancer Res 9:5418-5429 (2020). PubMed: 35117907