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重组RabMAb

重组Anti-S100A8抗体[EPR3554] - BSA and Azide free (ab271863)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
  • Immunoprecipitation - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
  • Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3554] to S100A8 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 568 Alexa Fluor® 647 Unconjugated

概述

  • 产品名称

    Anti-S100A8抗体[EPR3554] - BSA and Azide free
    参阅全部 S100A8 一抗

  • 描述

    兔单克隆抗体[EPR3554] to S100A8 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, IPmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human cervical carcinoma, breast carcinoma, tonsil and spleen tissues. IP: HL-60 cell lysate.

  • 常规说明

    ab271863 is the carrier-free version of ab92331.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271863于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.
IP
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.
IP
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.

  • 组织特异性

    Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes but are absent in normal tissue macrophages and lymphocytes. Under chronic inflammatory conditions, such as psoriasis and malignant disorders, also expressed in the epidermis. Found in high concentrations at local sites of inflammation or in the serum of patients with inflammatory diseases such as rheumatoid, cystic fibrosis, inflammatory bowel disease, Crohn's disease, giant cell arteritis, cystic fibrosis, Sjogren's syndrome, systemic lupus erythematosus, and progressive systemic sclerosis. Involved in the formation and deposition of amyloids in the aging prostate known as corpora amylacea inclusions. Strongly up-regulated in many tumors, including gastric, esophageal, colon, pancreatic, bladder, ovarian, thyroid, breast and skin cancers.

  • 序列相似性

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.

  • 细胞定位

    Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Calgranulin-A antibody
      • Calprotectin L1L subunit antibody
      • CFAG antibody
      • Cystic fibrosis antigen antibody
      • Leukocyte L1 complex light chain antibody
      • Migration inhibitory factor-related protein 8 antibody
      • MRP-8 antibody
      • N-terminally processed antibody
      • p8 antibody
      • Protein S100-A8 antibody
      • S100 calcium-binding protein A8 antibody
      • S100A8 antibody
      • S10A8_HUMAN antibody
      • Urinary stone protein band A antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labeling S100A8 with purified ab92331 at 1/500.

      Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

      Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).

    • Immunoprecipitation - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
      Immunoprecipitation - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)

      ab92331 (purified) at 1/30 immunoprecipitating S100A8 in HL-60 (Human promyelocytic leukemia cell line) cell lysate (Lane 1).

      Lane 2 - rabbit monoclonal IgG instead of ab92331 in HL-60 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

      Blocking/Dilution buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling S100A8 with unpurified ab92331.

      Detection: DAB staining.

      Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling S100A8 with unpurified ab92331. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labeling S100A8 with unpurified ab92331.

      Detection: DAB staining.

      Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling S100A8 with unpurified ab92331. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).

    • Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)
      Anti-S100A8 antibody [EPR3554] - BSA and Azide free (ab271863)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab271863？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab271863 尚未被引用在任何文献中。

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