重组Anti-S100A4抗体[EPR2761(2)] - BSA and Azide free (Capture) (ab281227)
![Sandwich ELISA - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (Capture) (ab281227)](https://www.abcam.cn/ps/products/281/ab281227/Images/ab281227-3-human-s100a4-elisa-kit-sandwich-elisa-standard-curve-3.jpg "Sandwich ELISA - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (Capture) (ab281227)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2761(2)] to S100A4 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-S100A4抗体[EPR2761(2)] - BSA and Azide free (Capture)
参阅全部 S100A4 一抗 -
描述
兔单克隆抗体[EPR2761(2)] to S100A4 - BSA and Azide free (Capture) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISA, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa cell lysate
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常规说明
ab281227 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human S100A4. The recommended pair for sandwich ELISA is:
Capture: ab281227, Human S100A4 Capture Antibody (unconjugated)
Detector: ab281077, Human S100A4 Detector Antibody (unconjugated)
The reference range value is 15.6 - 1000 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2761(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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KO cell lysates
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281227于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23250-94] to S100A4 - BSA and Azide free (ab281077).
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
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| 说明 |
|---|
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23250-94] to S100A4 - BSA and Azide free (ab281077). |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa. |
靶标
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组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. - Information by UniProt
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数据库链接
- Entrez Gene: 6275 Human
- Omim: 114210 Human
- SwissProt: P26447 Human
- Unigene: 654444 Human
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别名
- 18A2 antibody
- 42A antibody
- calcium Placental protein antibody
see all
图片
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Sandwich ELISA - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (Capture) (ab281227)Example of Human S100A4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Western blot - Anti-S100A4 antibody - BSA and Azide free (Capture) (ab281227)All lanes : Anti-S100A4 antibody [EPR2761(2)] (ab124805) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : S100A4 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab124805).
Lanes 1 - 2: Merged signal (red and green). Green - ab124805 observed at 11 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab124805 was shown to react with S100A4 in wild-type HeLa cells in Western blot with loss of signal observed in S100A4 knockout cell line ab265709 (S100A4 knockout cell lysate ab257046). Wild-type HeLa and S100A4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab124805 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Anti-S100A4 antibody - BSA and Azide free (Capture) (ab281227)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281227 尚未被引用在任何文献中。
