重组Anti-S100A11抗体[EPR11172] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (AB236123)

Intracellular Flow Cytometry analysis of PC-3 cells using ab180593 at a 1/150 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (AB236123)

ab180593 staining S100A11 in human thyroid carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraffin and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/3000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (AB236123)

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling S100A11 with ab180593 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (AB236123)

Immunofluorescence analysis of BxPC-3 cells (fixative 4% paraformaldehyde) labeling S100A11 with ab180593 at a 1/100 dilution (left image), and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (AB236123)

ab180593 staining S100A11 in PC-3 (human prostate adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/400. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 (1/1000) and ab150120 (1/1000) were used as counterstains for primary antibody ab180593 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1 : Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2 : Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (AB236123)

ab180593 staining S100A11 in PC-12 (rat adrenal gland pheochromocytoma) cell line by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/180. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control : Rabbit monoclonal IgG (Black)

Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).