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Anti-S100抗体[B32.1] (ab7852)

Reviews (3)Q&A (5)References (17)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-S100 antibody [B32.1] (ab7852)

    Key features and details

    • Mouse monoclonal [B32.1] to S100
    • Suitable for: IHC-FoFr
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-S100抗体[B32.1]
      参阅全部 S100 一抗

    • 描述

      小鼠单克隆抗体[B32.1] to S100

    • 宿主

      Mouse

    • 特异性

      This antibody reacts with alpha and beta subunits of human S-100 protein (21-24 kD).

    • 经测试应用

      适用于: IHC-FoFrmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Full length native protein (purified) corresponding to Cow S100.
      Database link: P02639

    • 阳性对照

      • Melanoma tissues containing nerves, such as intestine and pancreas. Brain tissue.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab7852于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-FoFr
    1/500 - 1/1000. 1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4.
    说明
    IHC-FoFr
    1/500 - 1/1000. 1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4.

    靶标

    • 功能

      Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

    • 组织特异性

      Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

    • 序列相似性

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.

    • 细胞定位

      Cytoplasm.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Bpb antibody
        • NEF antibody
        • Protein S100-A1 antibody
        • S-100 protein alpha chain antibody
        • S-100 protein subunit alpha antibody
        • S100 alpha antibody
        • S100 beta antibody
        • S100 calcium binding protein A1 antibody
        • S100 calcium binding protein B antibody
        • S100 calcium-binding protein A1 antibody
        • S100 protein alpha polypeptide antibody
        • S100A antibody
        • s100a1 antibody
        • S100B antibody
        • S100beta antibody
        • S10A1_HUMAN antibody
        see all

      图片

      • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-S100 antibody [B32.1] (ab7852)
        Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-S100 antibody [B32.1] (ab7852)

        ab7852 at a dilution of 1/1000, staining Astrocytes (purple; Alexa 488 secondary at 1/2000) on 30µm coronal brain section tissue in free floating IHC (see protocol link for detailed description). [A] Image taken with 20x objective and [B] Image taken with 40x objective. Images coloured in Photoshop.

        NB: No labeling observed following omission of primary antibody.

        Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

      文献 (17)

      发表研究结果有使用 ab7852？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab7852 被引用在 17 文献中.

      • Galiakberova AA  et al. IPSC-Derived Human Neurons with GCaMP6s Expression Allow In Vitro Study of Neurophysiological Responses to Neurochemicals. Neurochem Res 47:952-966 (2022). PubMed: 34855047
      • Gomez-Sintes R  et al. Targeting retinoic acid receptor alpha-corepressor interaction activates chaperone-mediated autophagy and protects against retinal degeneration. Nat Commun 13:4220 (2022). PubMed: 35864098
      • Arotcarena ML  et al. Pilot Study Assessing the Impact of Intrathecal Administration of Variants AAV-PHP.B and AAV-PHP.eB on Brain Transduction in Adult Rhesus Macaques. Front Bioeng Biotechnol 9:762209 (2021). PubMed: 34869273
      • Alhindi A  et al. Terminal Schwann cells at the human neuromuscular junction. Brain Commun 3:fcab081 (2021). PubMed: 33977269
      • Wu P  et al. Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair. Bioact Mater 6:3515-3527 (2021). PubMed: 33842738
      View all Publications for this product

      客户评价及客户问答

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      1-8 of 8 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [B32.1]

       Poor
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Dog Tissue sections (Skin tumor)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH 6.0)
      Permeabilization
      No
      Specification
      Skin tumor
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 20°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Oct 16 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [B32.1] - Astrocyte Marker

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Nerve)
      Specification
      Nerve
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citric acid
      Permeabilization
      No
      Blocking step
      non fat milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 4°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. 哲也 今村

      Verified customer

      提交于 Jul 17 2008

      Immunocytochemistry abreview for Anti-S100 antibody [B32.1] - Astrocyte Marker

       Average
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Rat Cultured Cells (BMSC (Bone Marrow Stem Cell))
      Specification
      BMSC (Bone Marrow Stem Cell)
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - Triton X100
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Apr 25 2008

      Question

       Dear technical support: This customer has highly interested in ab7852 (Anti-S100 antibody [B32.1] - Astrocyte Marker), this customer wants to know whether this antibody can recognize to the antigen ab54050 or not. If not, could you please help this customer to choice a suitable one for her? Thanks for your kindly assistance. Best regards

      Read More
      Answer

      Thank you for your inquiry. I can confirm that antibody ab7852 should recognize the protein ab54050. But ab7852 (Anti-S100 antibody [B32.1] - Astrocyte Marker) is not tested and guaranteed for WB and it is not known if it binds to denatured S100 beta protein. Therefore I cannot recommend to use ab7852 with ab54050 in WB. If your customer is looking for a positive control for ab7852 (Anti-S100 antibody [B32.1] - Astrocyte Marker), I can recommend to use melanoma tissues containing nerves, such as intestine and pancreas, and brain tissue. Antibodies against S100 human protein that can be used in ELISA and WB can be found using the following link: https://www.abcam.com/index.html?pageconfig=searchresults&search=S100&pt=1&sk=targ&sv=S100&sn=S100&l=4&fViewMore=1 If I misunderstood the intentions of your customer, please do not hesitate to contact me again.

      Read More

      Question

      I would like to buy a primary antibody product of yours, the ab7852 or the ab115968. Please let me know do the latter also react with either alpha or beta subunits of S-100 protein and what is the price of them with additional charges for shipping the products to Budapest, Hungary.

      Read More
      Answer

      Thank you for your inquiry. Yes, both ab7852 and ab115968 react with the alpha and beta subunits of S-100 protein. Anti-S100 antibody [B32.1] - Astrocyte Marker (ab7852) is €350.00 for 500ul. https://www.abcam.com/S100-antibody-B32-1-Astrocyte-Marker-ab7852.html Anti-S100 antibody [S100 217] (ab115968) is €360.00 for 1ml. https://www.abcam.com/S100-antibody-S100-217-ab115968.html Shipping & Handling is €63.00 to Hungary. You can order by: Phone: +44 (0)1223 696000 Email: orders@abcam.com Fax: +44 (0)1223 771600 I hope this information helps. Please contact us with any other questions.

      Read More

      Question

      When this antibody was tested in IHC-frozen sections, how were the sections fixed?

      Read More
      Answer

      Thank you for your enquiry and patience. The originator of ab7852 has informed me that the sections were fixed with formalin. Please contact us again if you have any additional questions.

      Read More

      Question

      Thank you for your very quick answer! I would have wanted to order a S100 beta specific antibody but the product ab7852 is an antibody against S100 protein. I want to use it as a specific marker of astrocytes or glial cells in the hippocampus and was wondering whether S100 protein could be detected in neurons as well. In other words could you certify that S100 protein detected by ab7852 antibody will label exclusively glial cells in the dentate gyrus of the hippocampus and not the neurons, evend immature neurons or neuronal precursor cells? Sorry for insisting and thank you again for your response.

      Read More
      Answer

      Thank you for your reply. I'm sorry I didn't see that ab7852 was against S100 and not S100 beta, thank you for noticing this. I cannot guarantee indeed that the protein will not be present in cells other than astrocytes of glial cells,the current literature on this protein may be able to provide this information. Please do not hesitate to contact me if you need any further help,

      Read More

      Question

      You list 1:50 for IHC in paraffin... is any antigen retrieval necessary? Do you suggest an enhanced secondary system such as ABC or a regular streptavidin reagent?

      Read More
      Answer

      Use enzymatic antigen retrieval using the protocol linked on the right hand side of the Abcam homepage. ABC is recommended as a visualisation technique.

      Read More

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