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Neuroscience Neurotransmission Calcium Signaling Calcium Binding Proteins

Anti-S100抗体[4C4.9] (ab4066)

  • Datasheet
  • SDS
Reviews (12)Q&A (11)References (66)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

Key features and details

  • Mouse monoclonal [4C4.9] to S100
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-S100抗体[4C4.9]
    参阅全部 S100 一抗
  • 描述

    小鼠单克隆抗体[4C4.9] to S100
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Full length protein. Purified bovine brain S-100 protein

  • 阳性对照

    • IHC-P: Human Melanoma and skin tissue.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 29/01/2021. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituent: Tissue culture supernatant
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    4C4.9
  • 同种型

    IgG2a
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class IV
    • Neurofilaments
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4066于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (4)
1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.
说明
IHC-P
1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.

靶标

  • 功能

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • 组织特异性

    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • 序列相似性

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession P23297 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6271 Human
    • Omim: 176940 Human
    • SwissProt: P23297 Human
    • Unigene: 515715 Human
    • 别名

      • Bpb antibody
      • NEF antibody
      • Protein S100-A1 antibody
      • S-100 protein alpha chain antibody
      • S-100 protein subunit alpha antibody
      • S100 alpha antibody
      • S100 beta antibody
      • S100 calcium binding protein A1 antibody
      • S100 calcium binding protein B antibody
      • S100 calcium-binding protein A1 antibody
      • S100 protein alpha polypeptide antibody
      • S100A antibody
      • s100a1 antibody
      • S100B antibody
      • S100beta antibody
      • S10A1_HUMAN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

      Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

      Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry of Formalin fixed paraffin embedded human skin labeling S100 with ab4066.

      This image was generated using the ascites version of the product.

    实验方案

    • IHC-FoFr protocol

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (66)

    发表研究结果有使用 ab4066?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4066 被引用在 66 文献中.

    • Qu H  et al. Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease. Diabetes 70:1372-1387 (2021). PubMed: 33741719
    • Hernández-Díaz N  et al. Parallel evolution of placental calcium transfer in the lizard Mabuya and eutherian mammals. J Exp Biol 224:N/A (2021). PubMed: 33568441
    • Wang Y  et al. Betacellulin regulates peripheral nerve regeneration by affecting Schwann cell migration and axon elongation. Mol Med 27:27 (2021). PubMed: 33794764
    • Omiya H  et al. BMP signaling suppresses Gemc1 expression and ependymal differentiation of mouse telencephalic progenitors. Sci Rep 11:613 (2021). PubMed: 33436697
    • Rinwa P  et al. Demise of nociceptive Schwann cells causes nerve retraction and pain hyperalgesia. Pain 162:1816-1827 (2021). PubMed: 33979318
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 23 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Melanoma suspicious cutaneous tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    Yes - H2O2
    Specification
    Melanoma suspicious cutaneous tissue
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 25 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Cerebellum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH6
    Permeabilization
    No
    Specification
    Cerebellum
    Blocking step
    Normal Donkey Serum (NDS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Estela Munoz

    Verified customer

    提交于 Mar 15 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (melanoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
    Permeabilization
    No
    Specification
    melanoma
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 19 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Other
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 14 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-S100 antibody [4C4.9]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain (Hippocampus))
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1%TritonX-100/PBS
    Specification
    Brain (Hippocampus)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5, 2 · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 14 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-S100 antibody [4C4.9] - Astrocyte Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Fixative
    Acetone
    Permeabilization
    Yes - 0,1% Saponin / PBS
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 27 2012

    Question

    IHC-FoFr with rat peripheral nerve
    sees no staining
    fix: 4% PFA perfused, postfixed with PFA
    no AR
    Ab: 1/100 o/n 4 degree; 1/50 o/n RT
    block: BSA + goat serum
    2nd: goat anti-mouse, OK

    suggested: try an enzymatic AR step

    Read More

    Abcam community

    Verified customer

    Asked on Feb 21 2013

    Answer


    As we discussed, trying an enzymatic antigen retrieval step could help with the signal.

    If not, please let me know.

    As for the protocol on the datasheet - this was added most likely from a collaborator or customer. Thus, these must be the conditions that were used by them. I still would suggest to do the overnight incubation at 4 degree as this makes more sense.

    Read More

    Abcam Scientific Support

    回复于 Feb 21 2013

    Question

    Dear Sir or Madam,

    could you please also provide me with the concentration of your product Anti-S100 antibody [4C4.9] - Astrocyte Marker (ab4066)?

    Best Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2012

    Answer

    Thank you for your interest in our products.

    The unit size of this antibody (ab4066) is 500 µl and the concentration is not determined since it is not affinity purified - only IgG fraction.

    If I can assist further, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Aug 10 2012

    Question

    Thank you for sending the replacement antibody. This one worked very well both in vitro using human induced pluripotent stem cells and in vivo using mouse spinal cord sections.

    Read More

    Abcam community

    Verified customer

    Asked on May 03 2012

    Answer

    That's great news! Thanks so much for letting us know.

    Please feel free to contact me if you need anything further.

    Read More

    Abcam Scientific Support

    回复于 May 03 2012

    Question

    I have used this antibody on astrocytes grown in culture in ICC and mousespinal cord via IHC-Fr. In neither application did I see any signal using 1:200 dilution of the antibody; using another antibody to an astrocyte marker protein, GFAP, is was able to detect astrocytes in both applications using the same samples.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 30 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1080427.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Apr 30 2012

    1-10 of 23 Abreviews or Q&A

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