重组Anti-S100 alpha抗体[EPR5251] (ab109384)
![Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)](https://www.abcam.cn/ps/products/109/ab109384/Images/ab109384-485181-anti-s100-alpha-antibody-epr5251-western-blot-c2c12-mouse.jpg "Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5251] to S100 alpha
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-S100 alpha抗体[EPR5251]
参阅全部 S100 alpha 一抗 -
描述
兔单克隆抗体[EPR5251] to S100 alpha -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: kidney, C2C12, mouse kidney, Human brain, HT-1080 and mouse heart lysate IF: HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 5.00 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5251 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109384于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
|
|
| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
|
ICC/IF
1/100 - 1/250. |
应用说明
Is unsuitable for IHC-P.
靶标
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功能
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
组织特异性
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
序列相似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 6271 Human
- Entrez Gene: 20193 Mouse
- Entrez Gene: 295214 Rat
- Omim: 176940 Human
- SwissProt: P23297 Human
- SwissProt: P56565 Mouse
- SwissProt: P35467 Rat
- Unigene: 515715 Human
see all -
别名
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
图片
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Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution + C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 60 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)](https://www.abcam.cn/ps/products/109/ab109384/Images/ab109384-122175-anti-s100-alpha-antibody-epr5251-western-blot.jpg)
Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution
Lane 1 : Rat kidney tissue lysate
Lane 2 : C2C12 cell lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Human brain lysate
Lane 5 : HT-1080 lysate
Lane 6 : Mouse heart tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384)](https://www.abcam.cn/ps/products/109/ab109384/Images/ab109384-122174-anti-s100-alpha-antibody-epr5251-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384)ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells -
![OI-RD Scanning - Anti-S100 alpha antibody [EPR5251] (ab109384)](https://www.abcam.cn/ps/products/109/ab109384/Images/ab109384-122173-anti-s100-alpha-antibody-epr5251.jpg)
OI-RD Scanning - Anti-S100 alpha antibody [EPR5251] (ab109384) -
![Anti-S100 alpha antibody [EPR5251] (ab109384)](https://www.abcam.cn/ps/products/109/ab109384/Images/ab109384-4-benefits-of-recombinant-antibodies.png)
Anti-S100 alpha antibody [EPR5251] (ab109384)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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