Anti-Ryanodine Receptor抗体[34C] (ab2868)
![Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [34C] (ab2868)](https://www.abcam.cn/ps/products/2/ab2868/Images/ab2868-6627-anti-ryanodine-receptor-antibody-34c-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [34C] (ab2868)")
Key features and details
- Mouse monoclonal [34C] to Ryanodine Receptor
- Suitable for: IHC-P, ICC/IF, IHC-Fr, IP, Inhibition Assay, WB
- Reacts with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human, Pig, Xenopus laevis, Non human primates
- Isotype: IgG1
概述
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产品名称
Anti-Ryanodine Receptor抗体[34C]
参阅全部 Ryanodine Receptor 一抗 -
描述
小鼠单克隆抗体[34C] to Ryanodine Receptor -
宿主
Mouse -
特异性
Detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms. In chickens, this antibody detects the alpha, beta and cardiac isoforms. This antibody also detects RyR-3 in mouse cells. In frog, this antibody detects the alpha and beta isoforms. In fish, this antibody detects the alpha isoform. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain results in intense staining of cerebellar Purkinje neurons. -
经测试应用
适用于: IHC-P, ICC/IF, IHC-Fr, IP, Inhibition Assay, WBmore details -
种属反应性
与反应: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human, Pig, Xenopus laevis, Non human primates
预测可用于: Fish, Amphibian
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免疫原
Full length protein corresponding to Chicken Ryanodine Receptor. Partially purified chicken pectoral muscle ryanodine receptor.
Database link: P21817-1 -
阳性对照
- rat skeletal muscle
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
Primary antibody说明
The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively. -
克隆
单克隆 -
克隆编号
34C -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC/IF | (6) |
Use a concentration of 1 µg/ml.
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| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| Inhibition Assay |
Use at an assay dependent concentration.
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| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 565 kDa.
As RyR is a large protein, we recommend using a 6-8% gel, wet transfering protein overnight at low voltage, adding 0.1% SDS to transfer buffer and reducing methanol to 10% or less. |
| 说明 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-Fr
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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Inhibition Assay
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 565 kDa. As RyR is a large protein, we recommend using a 6-8% gel, wet transfering protein overnight at low voltage, adding 0.1% SDS to transfer buffer and reducing methanol to 10% or less. |
靶标
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功能
Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis. -
组织特异性
Skeletal muscle and brain (cerebellum and hippocampus). -
疾病相关
Malignant hyperthermia 1
Central core disease of muscle
Multiminicore disease with external ophthalmoplegia
Myopathy, congenital, with fiber-type disproportion
Defects in RYR1 may be a cause of Samaritan myopathy, a congenital myopathy with benign course. Patients display severe hypotonia and respiratory distress at birth. Unlike other congenital myopathies, the health status constantly improves and patients are minimally affected at adulthood. -
序列相似性
Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily.
Contains 3 B30.2/SPRY domains.
Contains 5 MIR domains. -
结构域
The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum (SR) and the T-tubule. -
翻译后修饰
Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843.
Activated by reversible S-nitrosylation. Repeated very high-level exercise increases S-nitrosylation. -
细胞定位
Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 281473 Cow
- Entrez Gene: 403615 Dog
- Entrez Gene: 606491 Dog
- Entrez Gene: 6261 Human
- Entrez Gene: 6262 Human
- Entrez Gene: 6263 Human
- Entrez Gene: 20190 Mouse
- Entrez Gene: 20191 Mouse
see all -
别名
- Arrhythmogenic right ventricular dysplasia 2 antibody
- ARVC2 antibody
- ARVD2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [34C] (ab2868)
ICC/IF image of ab2868 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2868, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [34C] (ab2868)](https://www.abcam.cn/ps/products/2/ab2868/Images/ab2868-263154-anti-ryanodine-receptor-antibody-34c-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [34C] (ab2868)Image courtesy of an Abreview submitted by Jenny Sharpeab2868 staining Ryanodine Receptor (red) in Mouse Skeletal muscle cells at day 10 of agrin-treated differentation by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 10% serum for 45 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) for 2 hours at 20°C. An Alexa Fluor® 647-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Nucleus stained blue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [34C] (ab2868)](https://www.abcam.cn/ps/products/2/ab2868/Images/ab2868-6625-anti-ryanodine-receptor-antibody-34c-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [34C] (ab2868)Immunohistochemistry was performed on biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Ryanodine Receptor ab2868 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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![Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [34C] (ab2868)](https://www.abcam.cn/ps/products/2/ab2868/Images/ab2868-263156-anti-ryanodine-receptor-antibody-34c-immunofluorescence.png)
Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [34C] (ab2868)Image courtesy of an Abreview submitted by Can Zhouab2868 staining Ryanodine Receptor in Rat cardiomyocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1) and blocked with 3% BSA for 1 hour at 18°C. Samples were incubated with primary antibody (1/300 in PBS + 3% BSA) for 16 hours at 4°C. An Alexa Fluor® 546-conjugated Goat anti-mouse IgG (H+L) polyclonal (1/300) was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (75)
ab2868 被引用在 75 文献中.
- Song XW et al. Cardiovascular-specific PSEN1 deletion leads to abnormalities in calcium homeostasis. Cell Biol Int 46:475-487 (2022). PubMed: 34939719
- Salazar-Enciso R et al. Aldosterone-Induced Sarco/Endoplasmic Reticulum Ca2+ Pump Upregulation Counterbalances Cav1.2-Mediated Ca2+ Influx in Mesenteric Arteries. Front Physiol 13:834220 (2022). PubMed: 35360237
- Endo Y et al. Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility. Nat Commun 13:3403 (2022). PubMed: 35697689
- Chen L et al. Chai-Hu-San-Shen Capsule Ameliorates Ventricular Arrhythmia Through Inhibition of the CaMKII/FKBP12.6/RyR2/Ca2+ Signaling Pathway in Rats with Myocardial Ischemia. Evid Based Complement Alternat Med 2022:2670473 (2022). PubMed: 36225189
- Turovsky EA et al. Deregulation of Ca2+-Signaling Systems in White Adipocytes, Manifested as the Loss of Rhythmic Activity, Underlies the Development of Multiple Hormonal Resistance at Obesity and Type 2 Diabetes. Int J Mol Sci 22:N/A (2021). PubMed: 34065973