Anti-RXRG抗体(ab53162)
Key features and details
- Rabbit polyclonal to RXRG
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RXRG抗体 -
描述
兔多克隆抗体to RXRG -
宿主
Rabbit -
特异性
ab53162 detects endogenous levels of total Retinoic X Receptor gamma protein. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human RXRG.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab53162 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53162于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 2 - 4 µg/ml.
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说明 |
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IHC-P
Use a concentration of 2 - 4 µg/ml. |
靶标
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功能
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. -
序列相似性
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6258 Human
- Omim: 180247 Human
- SwissProt: P48443 Human
- Unigene: 26550 Human
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别名
- MGC109416 antibody
- NR2B3 antibody
- Nuclear receptor subfamily 2 group B member 3 antibody
see all
图片
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Ab53162 staining RXRG in human nucleus accumbens.
Left panel: with primary antibody at a dilution of 4µg/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH9.0 in a Dako PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab53162 被引用在 2 文献中.
- Kovalevich J et al. Cocaine induces nuclear export and degradation of neuronal retinoid X receptor-? via a TNF-a/JNK- mediated mechanism. J Neuroimmune Pharmacol 10:55-73 (2015). WB, ICC/IF ; Mouse . PubMed: 25586717
- Donmez G et al. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10. Cell 142:320-332 (2010). WB ; Mouse . PubMed: 20655472