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使用敲除细胞株进行验证

Anti-RUNX3抗体[R3-5G4] (ab40278)

Reviews (2)Q&A (1)References (30)
Western blot - Anti-RUNX3 antibody [R3-5G4] (ab40278)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX3 antibody [R3-5G4] (ab40278)
  • Flow Cytometry (Intracellular) - Anti-RUNX3 antibody [R3-5G4] (ab40278)
  • Western blot - Anti-RUNX3 antibody [R3-5G4] (ab40278)

Key features and details

  • Mouse monoclonal [R3-5G4] to RUNX3
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-RUNX3抗体[R3-5G4]
    参阅全部 RUNX3 一抗

  • 描述

    小鼠单克隆抗体[R3-5G4] to RUNX3

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment corresponding to Human RUNX3 aa 150 to the C-terminus (C terminal).

  • 阳性对照

    • ab40278 gave a positive result in the following whole cell lysates: Jurkat (Human T cell lymphoblast-like cell line) Raji (Human Burkitt's lymphoma cell line) SW480 (Human colon adenocarcinoma cell line)

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 克隆

    单克隆

  • 克隆编号

    R3-5G4

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab40278于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P (1)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.

  • 序列相似性

    Contains 1 Runt domain.

  • 结构域

    A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.

  • 翻译后修饰

    Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.

  • 细胞定位

    Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Acute myeloid leukemia 2 protein antibody
      • Acute myeloid leukemia gene 2 antibody
      • AML 2 antibody
      • AML2 antibody
      • CBF alpha 3 antibody
      • CBF-alpha-3 antibody
      • CBFA 3 antibody
      • CBFA3 antibody
      • Core binding factor alpha 3 subunit antibody
      • core binding factor antibody
      • Core binding factor runt domain alpha subunit 3 antibody
      • Core binding factor subunit alpha 3 antibody
      • core-binding factor antibody
      • Core-binding factor subunit alpha-3 antibody
      • Oncogene AML 2 antibody
      • Oncogene AML-2 antibody
      • PEA2 alpha C antibody
      • PEA2-alpha C antibody
      • PEBP2 alpha C antibody
      • PEBP2-alpha C antibody
      • Pebp2a3 antibody
      • PEBP2aC antibody
      • Polyomavirus enhancer binding protein 2 alpha C subunit antibody
      • Polyomavirus enhancer-binding protein 2 alpha C subunit antibody
      • runt domain alpha subunit 3 antibody
      • runt related transcription factor 3 antibody
      • Runt-related transcription factor 3 antibody
      • RUNX 3 antibody
      • Runx3 antibody
      • RUNX3_HUMAN antibody
      • SL3 3 enhancer factor 1 alpha C subunit antibody
      • SL3-3 enhancer factor 1 alpha C subunit antibody
      • SL3/AKV core binding factor alpha C subunit antibody
      • SL3/AKV core-binding factor alpha C subunit antibody
      • Transcription factor AML2 antibody
      see all

    图片

    • Western blot - Anti-RUNX3 antibody [R3-5G4] (ab40278)
      Western blot - Anti-RUNX3 antibody [R3-5G4] (ab40278)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: RUNX3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab40278 observed at 44 kDa. Red - loading control, ab176560, observed at 50 kDa.

      ab40278 was shown to specifically react with RUNX3 in wild-type HAP1 cells as signal was lost in RUNX3 knockout cells. Wild-type and RUNX3 knockout samples were subjected to SDS-PAGE. ab40278 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX3 antibody [R3-5G4] (ab40278)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX3 antibody [R3-5G4] (ab40278)
      IHC image of RUNX3 staining in human lymphoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40278, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    • Flow Cytometry (Intracellular) - Anti-RUNX3 antibody [R3-5G4] (ab40278)
      Flow Cytometry (Intracellular) - Anti-RUNX3 antibody [R3-5G4] (ab40278)
      Overlay histogram showing HEK293 cells stained with ab40278 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40278, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Western blot - Anti-RUNX3 antibody [R3-5G4] (ab40278)
      Western blot - Anti-RUNX3 antibody [R3-5G4] (ab40278)
      All lanes : Anti-RUNX3 antibody [R3-5G4] (ab40278) at 1 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 3 : SW480 whole cell lysate (ab3957)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Rabbit Anti-Mouse IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 44 kDa
      Additional bands at: 46 kDa (possible isoform)

    文献 (30)

    发表研究结果有使用 ab40278？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab40278 被引用在 30 文献中.

    • Douchi D  et al. A Point Mutation R122C in RUNX3 Promotes the Expansion of Isthmus Stem Cells and Inhibits Their Differentiation in the Stomach. Cell Mol Gastroenterol Hepatol 13:1317-1345 (2022). PubMed: 35074568
    • Lee SH  et al. RUNX3 methylation drives hypoxia-induced cell proliferation and antiapoptosis in early tumorigenesis. Cell Death Differ 28:1251-1269 (2021). PubMed: 33116296
    • Carrato C  et al. Glioblastoma TCGA Mesenchymal and IGS 23 Tumors are Identifiable by IHC and have an Immune-phenotype Indicating a Potential Benefit from Immunotherapy. Clin Cancer Res 26:6600-6609 (2020). PubMed: 32998960
    • Xiao Z  et al. RUNX3 inhibits the invasion and migration of esophageal squamous cell carcinoma by reversing the epithelial-mesenchymal transition through TGF-ß/Smad signaling. Oncol Rep 43:1289-1299 (2020). PubMed: 32323849
    • Liu H  et al. Inhibition of PIM1 attenuates the stem cell-like traits of breast cancer cells by promoting RUNX3 nuclear retention. J Cell Mol Med 24:6308-6323 (2020). PubMed: 32307917
    View all Publications for this product

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RUNX3 antibody [R3-5G4]

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (stomach)
    Specification
    stomach
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA (pH 9.0)
    Permeabilization
    No
    Blocking step
    RE7102, NovoLink Polymer Detection System as blocking agent for 5 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Yuan LI

    Verified customer

    提交于 Aug 20 2010

    ChIP abreview for Anti-RUNX3 antibody [R3-5G4]

     Poor
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (purified CD34+ cells)
    Specification
    purified CD34+ cells
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 5 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 0.4% formaldehyde
    Detection step
    Real-time PCR
    Positive control
    known bound region
    Negative control
    known neg control region
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 06 2009

    Question

    Testing disocunt code to try in IF.

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    Answer

    DISCOUNT CODE: ***********
    Expiration date: ***********

    I am very pleased to hear you would like to accept our offer and test ab40278 in IF code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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