Anti-RUNX2抗体[2B9] (ab76956)
![Western blot - Anti-RUNX2 antibody [2B9] (ab76956)](https://www.abcam.cn/ps/products/76/ab76956/Images/ab76956-92543-anti-runx2-antibody-western-blot.jpg "Western blot - Anti-RUNX2 antibody [2B9] (ab76956)")
Key features and details
- Mouse monoclonal [2B9] to RUNX2
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG2a
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RUNX2抗体[2B9]
参阅全部 RUNX2 一抗 -
描述
小鼠单克隆抗体[2B9] to RUNX2 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, Flow Cyt, WBmore details -
种属反应性
与反应: Rat, Human, Recombinant fragment
预测可用于: Horse, Dog, Pig, Chimpanzee, Rhesus monkey
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免疫原
Recombinant fragment: NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA, corresponding to amino acids 251-351 of Human RUNX2 (NP_004339) with tag.
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阳性对照
- PC12 cell lysate;
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常规说明
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from Cell Culture supernatant. -
克隆
单克隆 -
克隆编号
2B9 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76956于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa. |
靶标
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功能
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. -
组织特异性
Specifically expressed in osteoblasts. -
疾病相关
Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies. -
序列相似性
Contains 1 Runt domain. -
结构域
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites. -
翻译后修饰
Phosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 472017 Chimpanzee
- Entrez Gene: 449531 Dog
- Entrez Gene: 100033890 Horse
- Entrez Gene: 860 Human
- Entrez Gene: 100155806 Pig
- Entrez Gene: 367218 Rat
- Omim: 600211 Human
- SwissProt: Q9XSB7 Horse
see all -
别名
- Acute myeloid leukemia 3 protein antibody
- Alpha subunit 1 antibody
- AML3 antibody
see all
图片
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Western blot - Anti-RUNX2 antibody [2B9] (ab76956)Anti-RUNX2 antibody [2B9] (ab76956) at 1 µg/ml + Recombinant tagged human RUNX2 fragment at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Predicted band size: 57 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (ab76956)](https://www.abcam.cn/ps/products/76/ab76956/Images/ab76956-92544-anti-runx2-antibody-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (ab76956)ab76956 at 10µg/ml staining RUNX2 in HeLa cells by Immunofluorescence.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-RUNX2 antibody [2B9] (ab76956)](https://www.abcam.cn/ps/products/76/ab76956/Images/ab76956-92541-anti-runx2-antibody-flow-cytometry.jpg)
Flow Cytometry - Anti-RUNX2 antibody [2B9] (ab76956)Overlay histogram showing Saos 2 cells stained with ab76956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
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![Western blot - Anti-RUNX2 antibody [2B9] (ab76956)](https://www.abcam.cn/ps/products/76/ab76956/Images/ab76956-92542-anti-runx2-antibody-western-blot.jpg)
Western blot - Anti-RUNX2 antibody [2B9] (ab76956)Anti-RUNX2 antibody [2B9] (ab76956) at 1 µg/ml + PC12 cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (324)
ab76956 被引用在 324 文献中.
- Luo J et al. Cerium Oxide Nanoparticles Promote Osteoplastic Precursor Differentiation by Activating the Wnt Pathway. Biol Trace Elem Res 201:865-873 (2023). PubMed: 35230639
- Huang X et al. Melatonin suppresses bone marrow adiposity in ovariectomized rats by rescuing the imbalance between osteogenesis and adipogenesis through SIRT1 activation. J Orthop Translat 38:84-97 (2023). PubMed: 36381247
- Sun B et al. Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta. J Orthop Translat 38:175-189 (2023). PubMed: 36439629
- Xie W et al. Herpud1 deficiency alleviates homocysteine-induced aortic valve calcification. Cell Biol Toxicol 39:2665-2684 (2023). PubMed: 36746840
- Guo K et al. Long Non-coding RNA KTN1-AS1 Targets miR-505 to Promote Glioblastoma Progression. Behav Neurol 2023:4190849 (2023). PubMed: 36762036