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AB76956

Anti-RUNX2 抗体 [2B9]

Anti-RUNX2 antibody [2B9]

4

(8 Reviews)

|

(409 Publications)

Anti-RUNX2 antibody [2B9] (ab76956) is a mouse monoclonal antibody detecting RUNX2 in Western Blot, Flow Cytometry, ICC/IF. Suitable for Human, Rat,.

- Over 320 publications
- Trusted since 2009

查看别名

AML3, CBFA1, OSF2, PEBP2A, RUNX2, Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A

3 Images
Flow Cytometry - Anti-RUNX2 antibody [2B9] (AB76956)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-RUNX2 antibody [2B9] (AB76956)

Overlay histogram showing Saos 2 cells stained with ab76956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76956, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (AB76956)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (AB76956)

ab76956 at 10μg/ml staining RUNX2 in HeLa cells by Immunofluorescence.

This image was generated using the ascites version of the product.

Western blot - Anti-RUNX2 antibody [2B9] (AB76956)
  • WB

Unknown

Western blot - Anti-RUNX2 antibody [2B9] (AB76956)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-RUNX2 antibody [2B9] (ab76956) at 1 µg/mL

All lanes:

PC12 cell lysate at 25 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 57 kDa

Observed band size: 56 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

2B9

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

Yes

反应种属

Rat, Human

应用

ICC/IF, Flow Cyt, WB

applications

免疫原

Recombinant Fragment Protein within Human RUNX2 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q13950

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替代产品

Primary Antibodies

AB236639

Anti-RUNX2 antibody [EPR22858-106] - ChIP Grade

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR22858-106] - ChIP Grade (AB236639)

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Primary Antibodies

AB215954

Alexa Fluor® 488 Anti-RUNX2 antibody [EPR14334]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-RUNX2 antibody [EPR14334] (AB215954)

  • 0
  • 1
Primary Antibodies

AB215955

Alexa Fluor® 647 Anti-RUNX2 antibody [EPR14334]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RUNX2 antibody [EPR14334] (AB215955)

  • 0
  • 0
Primary Antibodies

AB192256

Anti-RUNX2 antibody [EPR14334]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR14334] (AB192256)

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Primary Antibodies

AB264077

Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (AB264077)

  • 0
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Primary Antibodies

AB240329

Anti-RUNX2 antibody [EPR14334] - BSA and Azide free

Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR14334] - BSA and Azide free (AB240329)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB93876

Anti-Osteocalcin antibody

Western blot - Anti-Osteocalcin antibody (AB93876)

  • 3
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Primary Antibodies

AB209484

Anti-Sp7 / Osterix antibody [EPR21034]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (AB209484)

  • 5
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Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
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Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

  • 4
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Product Specifications
Anti-RUNX2 antibody [2B9] (ab76956) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, WB in human, rat, samples.
Anti-RUNX2 antibody [2B9] (ab76956) specifically detects RUNX2 (UniProt ID: Q13950; Molecular weight: 57kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-RUNX2 antibody [2B9] (ab76956) has high sensitivity and specificity.
Anti-RUNX2 antibody [2B9] (ab76956) has been cited over 324 times in peer reviewed journals and is trusted by the scientific community.
Anti-RUNX2 antibody [2B9] (ab76956) has 7 independent reviews from customers.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
纯化说明
Purified from Cell Culture supernatant.
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis (PubMed : 28505335, PubMed : 28703881, PubMed : 28738062). Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation : synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.
See full target information RUNX2
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文献 (409)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 13:1639117 PubMed40837020

2025

Ipriflavone ameliorates intervertebral disc degeneration by inhibiting osteoporosis of vertebral body and pyroptosis of the nucleus pulposus in instability of lumbar spine and diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Sun,Hai-Jing Zhang,Hui Wang,Gang Ji,Ya-Heng Zhao,Gao-Cen Li,Shao-Shi Guo,Lu-Feng Lin,Yu-Jie Jin,Xue-Li Zhang,Xin-Yu Nan,Chang-Cheng Liu,Guo-Bin Liu

Materials today. Bio 34:102144 PubMed40791800

2025

Study on mRNA delivery via GelMA hydrogel-encapsulated extracellular vesicles for enhanced bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Li,Yueyang Sheng,Yi You,Ying Wang,Yanzhuo Zhang,Jianfeng Tao,Chengai Wu,Xu Jiang

Small science 5:2400468 PubMed40657193

2025

Magnetoactive Nanotopography on Hydrogels for Stimulated Cell Adhesion and Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Md Shariful Islam,Thomas G Molley,Gagan K Jalandhra,Jason Fang,Jamie J Kruzic,Kristopher A Kilian

Journal of cellular and molecular medicine 29:e70703 PubMed40629256

2025

Hypoxia-Driven Regulation of Osteogenic Differentiation in Human Periosteal Stem Cells via the HIF-1α/miR-129-5p/BMP2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Lu,Xinyu Wang,Nan Lu,Aimin Chen,Lianbo Xiao

Nature communications 16:6200 PubMed40615395

2025

Divide-and-conquer strategy with engineered ossification center organoids for rapid bone healing through developmental cell recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Xianzhu Zhang,Wei Jiang,Xinyu Wu,Chang Xie,Yi Zhang,Liying Li,Yuqing Gu,Zihao Hu,Xinrang Zhai,Renjie Liang,Tao Zhang,Wei Sun,Jinchun Ye,Wei Wei,Xiaozhao Wang,Yi Hong,Shufang Zhang,Youzhi Cai,Xiaohui Zou,Yihe Hu,Hongwei Ouyang

Cell biology and toxicology 41:99 PubMed40493284

2025

SENP3 alleviates osteoporosis via promoting SIRT3 transcription through the increase of DLX2 stability via SUMO2/3.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Bu,Xuezheng Xu,Yi Luo,Jianfan Liu,Feng Zhou

Scientific reports 15:19979 PubMed40481141

2025

EIF4A3 enhances the viability, invasion and osteogenic differentiation of BMSCs via the USP53/SMAD5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Cheng,Xiaoling Yang,Yongtao Tang,Changjun Wu,Zulin Tang,Siqi Wei

Medicina (Kaunas, Lithuania) 61: PubMed40428778

2025

Investigating the Associations Between Hmga2 Overexpression, R-Loop Reduction, and Bone Loss in Aging Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Cao,Yantong Wang,Dengsheng Xia

Folia histochemica et cytobiologica 63:65-78 PubMed40421821

2025

MiR-494-3p knockdown promotes osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomised rats by targeting Sirt1/TLR4/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianping Lei,Song Guo,Jiabing Kuang,Bo Shen,Zixi Li

Bioactive materials 49:608-626 PubMed40212781

2025

Continuous mechanical-gradient hydrogel with on-demand distributed Mn/Mg-doped hydroxyapatite@FeO for functional osteochondral regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei Xu,Yi Cui,Ping Li,Xuemei Sun,Zhiheng Chen,Jingxi Wang,Xuenan Gu,Xiaogang Wang,Yubo Fan
View all publications
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