AB124269

Anti-RUNX1T1/ETO/CDR抗体

Name: RUNX1T1/ETO/CDR抗体 (ab124269)| Abcam中文官网
Brand: Abcam Limited
SKU: AB124269
Price: 4606 CNY
Availability: InStock

Anti-RUNX1T1/ETO/CDR antibody

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(5 Publications)

Rabbit Polyclonal RUNX1T1/ETO/CDR antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RUNX1T1 aa 550 to C-terminus.

查看别名

AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2, RUNX1T1, Protein CBFA2T1, Cyclin-D-related protein, Eight twenty one protein, Protein ETO, Protein MTG8, Zinc finger MYND domain-containing protein 2

2 Images

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Immunoprecipitation - Anti-RUNX1T1/ETO/CDR antibody (AB124269)

ab124269 at 1 μg/ml staining RUNX1T1/ETO/CDR in 293T whole cell lysate immunoprecipitated using ab124269 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2 : control IgG. Predicted band size : 68 kDa. Exposure time : 30 seconds

All lanes:

Immunoprecipitation - Anti-RUNX1T1/ETO/CDR antibody (ab124269)

Predicted band size: 68 kDa

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Western blot - Anti-RUNX1T1/ETO/CDR antibody (AB124269)

All lanes:

Western blot - Anti-RUNX1T1/ETO/CDR antibody (ab124269) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Predicted band size: 68 kDa

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Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human RUNX1T1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q06455

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab124269 was affinity purified using an epitope specific to RUNX1T1/ETO/CDR immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RUNX1T1 also known as ETO or CDR is an important transcriptional regulator. It has a mass of approximately 70 kDa. This protein is expressed in various body tissues including hematopoietic cells. RUNX1T1 is heavily involved in transcriptional repression playing a role in chromatin remodeling and gene expression regulation. Structurally it binds to DNA through specific interactions which influences gene activation or repression contributing to the regulation of cell differentiation.

Biological function summary

The functions of RUNX1T1 involve its participation in the formation of transcriptional complexes. It interacts with several co-repressors and co-activators affecting gene transcription which is important for hematopoiesis. It regulates a range of gene expressions minimally necessary for normal blood cell development. These complexes enable RUNX1T1 to function in a tightly controlled way supporting tissue-specific gene expression and ensuring balance in cell proliferation and differentiation.

Pathways

RUNX1T1 has pivotal roles in the hematopoietic and differentiation pathways. It interacts with RUNX1 forming a RUNX1-RUNX1T1 fusion that disrupts normal blood cell development. This fusion alters its typical role in the regulation of gene transcription which intersects with other signaling pathways such as the TGF-beta pathway. RUNX1T1-ETO fusion is linked with important proteins involved in signaling and transcription processes which demonstrate its broad relevance within cellular growth pathways.

RUNX1T1's misregulation or fusion with RUNX1 characterizes certain leukemias notably acute myeloid leukemia (AML). This fusion protein acts aberrantly in hematopoiesis contributing to the development of leukemic cells. Apart from leukemia abnormalities in RUNX1T1 function can also impact other disorders related with defects in blood cell development. The disease association highlights RUNX1T1's interaction with other oncogenic proteins like FLT3 influencing AML pathogenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed : 10688654, PubMed : 12559562, PubMed : 15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed : 23251453). Can repress transactivation mediated by TCF12 (PubMed : 16803958). Acts as a negative regulator of adipogenesis (By similarity). The AML1-MTG8/ETO fusion protein frequently found in leukemic cells is involved in leukemogenesis and contributes to hematopoietic stem/progenitor cell self-renewal (PubMed : 23812588).

See full target information RUNX1T1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106701 PubMed37207277

2023

Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Han H Hsu,Greta Pintacuda,Ruize Liu,Eugeniu Nacu,April Kim,Kalliopi Tsafou,Natalie Petrossian,William Crotty,Jung Min Suh,Jackson Riseman,Jacqueline M Martin,Julia C Biagini,Daya Mena,Joshua K T Ching,Edyta Malolepsza,Taibo Li,Tarjinder Singh,Tian Ge,Shawn B Egri,Benjamin Tanenbaum,Caroline R Stanclift,Annie M Apffel,Steven A Carr,Monica Schenone,Jake Jaffe,Nadine Fornelos,Hailiang Huang,Kevin C Eggan,Kasper Lage

Cancer management and research 12:783-792 PubMed32099474

2020

Oncogenic Roles Of A Histone Methyltransferase SETDB2 In AML1-ETO Positive AML.

Applications

Unspecified application

Species

Unspecified reactive species

Guangfu Mu,Fangping Chen

Experimental physiology 105:75-87 PubMed31645082

2019

Long non-coding RNA EPB41L4A-AS2 suppresses progression of ovarian cancer by sequestering microRNA-103a to upregulate transcription factor RUNX1T1.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Sun,Peng Yang,Yunbin Gao

Haematologica 104:1597-1607 PubMed30792202

2019

RUNX1 inhibits proliferation and induces apoptosis of t(8;21) leukemia cells KLF4-mediated transactivation of P57.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Liu,Yanyan Xing,Wenting Lu,Shouyun Li,Zheng Tian,Haiyan Xing,Kejing Tang,Yingxi Xu,Qing Rao,Min Wang,Jianxiang Wang

Oncotarget 8:39994-40005 PubMed28611288

2017

An AML1-ETO/miR-29b-1 regulatory circuit modulates phenotypic properties of acute myeloid leukemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sayyed K Zaidi,Andrew W Perez,Elizabeth S White,Jane B Lian,Janet L Stein,Gary S Stein

View all publications

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Anti-RUNX1T1/ETO/CDR抗体

Anti-RUNX1T1/ETO/CDR antibody

Immunoprecipitation - Anti-RUNX1T1/ETO/CDR antibody (AB124269)

Western blot - Anti-RUNX1T1/ETO/CDR antibody (AB124269)