重组Anti-RUNX1 / AML1抗体[EPR23309-113] - ChIP Grade (ab272456)
![ChIC/CUT&RUN sequencing - Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)](https://www.abcam.cn/ps/products/272/ab272456/Images/ab272456-519241-anti-runx1-aml1-antibody-epr23309-113-chip-grade-chic-cut-and-run-sequencing-.jpg "ChIC/CUT&RUN sequencing - Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23309-113] to RUNX1 / AML1 - ChIP Grade
- Suitable for: ChIC/CUT&RUN-seq, Flow Cyt (Intra), WB, ChIP, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RUNX1 / AML1抗体[EPR23309-113] - ChIP Grade
参阅全部 RUNX1 / AML1 一抗 -
描述
兔单克隆抗体[EPR23309-113] to RUNX1 / AML1 - ChIP Grade -
宿主
Rabbit -
经测试应用
适用于: ChIC/CUT&RUN-seq, Flow Cyt (Intra), WB, ChIP, IPmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat, MOLT-4 and THP-1 whole cell lysates. Flow Cyt (intra): Jurkat cells. IP: Jurkat and K562 whole cell lysate. ChIP: Chromatin prepared from K562 cells. ChIC/CUT&RUN-Seq: K-562 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23309-113 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272456于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
5µg |
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| Flow Cyt (Intra) |
1/500.
|
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| WB |
1/1000. Detects a band of approximately 27-48 kDa (predicted molecular weight: 48 kDa).
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| ChIP | (1) |
Use 5 µg for 25 µg of chromatin.
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| IP |
1/30.
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| 说明 |
|---|
|
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. 5µg |
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Flow Cyt (Intra)
1/500. |
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WB
1/1000. Detects a band of approximately 27-48 kDa (predicted molecular weight: 48 kDa). |
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ChIP
Use 5 µg for 25 µg of chromatin. |
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IP
1/30. |
靶标
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功能
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation. -
组织特异性
Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood. -
疾病相关
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.
Note=A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3'-end of TEL to the alternate 5'-exon of AML-1H.
Note=A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.
Defects in RUNX1 are the cause of familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]. FPDMM is an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
Note=A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16. -
序列相似性
Contains 1 Runt domain. -
结构域
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes. -
翻译后修饰
Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with MYST3.
Methylated. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 861 Human
- Omim: 151385 Human
- SwissProt: Q01196 Human
- Unigene: 149261 Human
- Unigene: 612648 Human
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别名
- Acute myeloid leukemia 1 antibody
- Acute myeloid leukemia 1 protein antibody
- alpha subunit core binding factor antibody
see all
图片
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ChIC/CUT&RUN sequencing - Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5µg of ab272456 [EPR23309-113]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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![ChIP - Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)](https://www.abcam.cn/ps/products/272/ab272456/Images/ab272456-391008-anti-runx1-anml1-antibosy-epr23309-113-chip.jpg)
ChIP - Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)Chromatin was prepared from K-562 cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab272456 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are from paper PMID：20959602*https://www.abcam.com/resources?keywords=X%20ChIP%20protocol
The RUNX1 enrichment profile is consistent with what have been described in literature (PMID: 20959602).
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![Western blot - Anti-RUNX1 / AML1 antibody [EPR23309-113] (ab272456)](https://www.abcam.cn/ps/products/272/ab272456/Images/ab272456-390866-anti-runx1aml1-antibody-epr23309113-western-blot-jurkat.jpg)
Western blot - Anti-RUNX1 / AML1 antibody [EPR23309-113] (ab272456)All lanes : Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 48 kDa
Observed band size: 27-48 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
RUNX1 has several isoforms. The molecular weight observed is consistent with what have been described in literature (PMID:17431130, 29296779).
Exposure time: 3 minutes
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![Immunoprecipitation - Anti-RUNX1 / AML1 antibody [EPR23309-113] (ab272456)](https://www.abcam.cn/ps/products/272/ab272456/Images/ab272456-2-anti-runx1aml1-antibody-epr23309113-immunoprecipitation-jurkat.jpg)
Immunoprecipitation - Anti-RUNX1 / AML1 antibody [EPR23309-113] (ab272456)RUNX1 / AML1 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab272456 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab272456 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate 20 ug
Lane 2: ab272456 IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272456 in Jurkat whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
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![Flow Cytometry (Intracellular) - Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)](https://www.abcam.cn/ps/products/272/ab272456/Images/ab272456-1-anti-runx1aml1-antibody-epr23309113-flow-cytometry-jurkat.jpg)
Flow Cytometry (Intracellular) - Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling RUNX1 / AML1 with ab272456 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)](https://www.abcam.cn/ps/products/272/ab272456/Images/ab272456-391365-AntiRUNX1-AML1-antibody-EPR23309113-ChIP-Grade-immunoprecipitation-k562-human.jpg)
Immunoprecipitation - Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)RUNX1 / AML1 was immunoprecipitated from K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab272456 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272456 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: K562 whole cell lysate 10 µg
Lane 2: ab272456 IP in K562 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272456 in K562 whole cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.
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![Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)](https://www.abcam.cn/ps/products/272/ab272456/Images/ab272456-6-benefits-of-recombinant-antibodies.png)
Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade (ab272456)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (5)
ab272456 被引用在 5 文献中.
- Liu Y et al. RUNX1 Upregulation Causes Mitochondrial Dysfunction via Regulating the PI3K-Akt Pathway in iPSC from Patients with Down Syndrome. Mol Cells 46:219-230 (2023). PubMed: 36625318
- Xu J et al. Bioinformatics analysis of downstream circRNAs and miRNAs regulated by Runt-related transcription factor 1 in papillary thyroid carcinoma. Gland Surg 11:868-881 (2022). PubMed: 35694090
- Yuan ZL et al. Activation of GDNF-ERK-Runx1 signaling contributes to P2X3R gene transcription and bone cancer pain. iScience 25:104936 (2022). PubMed: 36072549
- Miao Y et al. lncRNA GAS5, as a ceRNA, inhibits the proliferation of diffuse large B-cell lymphoma cells by regulating the miR-18a-5p/RUNX1 axis. Int J Oncol 59:N/A (2021). PubMed: 34698360
- Zhang L et al. Targeting miR-126 in inv(16) acute myeloid leukemia inhibits leukemia development and leukemia stem cell maintenance. Nat Commun 12:6154 (2021). PubMed: 34686664