重组Anti-RUNX1 / AML1 + RUNX3 + RUNX2抗体[EPR3098] (ab133261)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3098] to RUNX1 / AML1 + RUNX3 + RUNX2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-RUNX1 / AML1 + RUNX3 + RUNX2抗体[EPR3098]
参阅全部 RUNX1 / AML1+RUNX3+RUNX2 一抗 -
描述
兔单克隆抗体[EPR3098] to RUNX1 / AML1 + RUNX3 + RUNX2 -
宿主
Rabbit -
特异性
The immunogen of this antibody is 100% homologous to mouse RUNX2 sequence. -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human RUNX1/ AML1 aa 150-250. The exact sequence is proprietary.
Database link: Q01196 -
阳性对照
- Molt 4 cell lysate, Human fetal thymus tissue lysate, Mouse thymus tissue lysate, Human tonsil tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3098 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133261于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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细胞定位
RUNX1 / AML1: Nucleus. RUNX3: Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm. RUNX2: Nucleus. -
数据库链接
- Entrez Gene: 860 Human
- Entrez Gene: 861 Human
- Entrez Gene: 864 Human
- Entrez Gene: 12393 Mouse
- Entrez Gene: 12394 Mouse
- Entrez Gene: 12399 Mouse
- Omim: 151385 Human
- Omim: 600210 Human
see all
图片
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All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261) at 1/1000 dilution
Lane 1 : Myc-DDK-tagged Human RUNX1 recombinant protein
Lane 2 : His-tagged Human RUNX2 recombinant protein
Lane 3 : His-tagged Human RUNX3 recombinant protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48.5 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer: 5% NFDM/TBST -
All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261) at 1/1000 dilution
Lane 1 : Molt 4 cell lysate
Lane 2 : Human fetal thymus lysate
Lane 3 : Mouse thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 48 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling RUNX1 / AML1 with ab133261 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)
Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using ab133261 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)
Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133261 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)
Immunohistochemical analysis of paraffin embedded normal Human heart tissue using ab133261 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)
Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133261 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)
Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133261 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133261 被引用在 1 文献中.
- Benoit YD et al. Sam68 Allows Selective Targeting of Human Cancer Stem Cells. Cell Chem Biol 24:833-844.e9 (2017). PubMed: 28648376