Anti-RSPO1抗体(ab106556)
Key features and details
- Rabbit polyclonal to RSPO1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RSPO1抗体
参阅全部 RSPO1 一抗 -
描述
兔多克隆抗体to RSPO1 -
宿主
Rabbit -
特异性
At least three isoforms of RSPO1 are known to exist; ab106556 will detect the largest and smallest isoforms. ab106556 will not cross-react with other RSPO family members. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human RSPO1 (N terminal).
Database link: Q2MKA7 -
阳性对照
- IHC-P: Human spleen and heart tissue. WB: Human spleen tissue, heart and Daudi cell lysate. Mouse 3T3/NIH cell lysate.
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常规说明
Antibodies should not be exposed to prolonged high temperatures.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106556于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa.
|
| IHC-P |
Use a concentration of 2.5 - 20 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa. |
|
IHC-P
Use a concentration of 2.5 - 20 µg/ml. |
靶标
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功能
Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. -
组织特异性
Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen. -
疾病相关
Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. -
序列相似性
Belongs to the R-spondin family.
Contains 2 FU (furin-like) repeats.
Contains 1 TSP type-1 domain. -
结构域
The FU repeats are required for activation and stabilization of beta-catenin. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 284654 Human
- Entrez Gene: 192199 Mouse
- Omim: 609595 Human
- SwissProt: Q2MKA7 Human
- SwissProt: Q9Z132 Mouse
- Unigene: 135015 Human
- Unigene: 42202 Mouse
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别名
- CRISTIN3 antibody
- FLJ40906 antibody
- hRspo1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO1 antibody (ab106556)
Immunohistochemistry of 4% paraformaldehyde-fixed human spleen labeling RSPO1 with 5171 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).
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Western blot - Anti-RSPO1 antibody (ab106556)Lane 1 : Anti-RSPO1 antibody (ab106556) at 0.5 µg
Lane 2 : Anti-RSPO1 antibody (ab106556) at 1 µg
All lanes : Human spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa -
Western blot - Anti-RSPO1 antibody (ab106556)All lanes : Anti-RSPO1 antibody (ab106556) at 2 µg
Lane 1 : Daudi cell lysate
Lane 2 : 3T3/NIH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa -
Western blot - Anti-RSPO1 antibody (ab106556)Lane 1 : Anti-RSPO1 antibody (ab106556) at 1 µg/ml
Lane 2 : Anti-RSPO1 antibody (ab106556) at 2 µg/ml
All lanes : Human heart tissue lysate
Predicted band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO1 antibody (ab106556)Immunohistochemical analysis of PFA fixed paraffin embedded Human spleen tissue sections labelling RSPO1 with ab106556, at 2.5 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO1 antibody (ab106556)Immunohistochemical analysis of PFA fixed paraffin embedded Human heart tissue sections labelling RSPO1 with ab106556, at 2.5 µg/mL.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab106556 被引用在 2 文献中.
- Chen X et al. Rspo1-LGR4 axis in BMSCs protects bone against radiation-induced injury through the mTOR-dependent autophagy pathway. J Cell Physiol 236:4273-4289 (2021). PubMed: 33452710
- Shi GX et al. Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. Int J Mol Sci 18:N/A (2017). WB ; Mouse . PubMed: 28272338