Anti-RSPH4A抗体(ab122157)
Key features and details
- Rabbit polyclonal to RSPH4A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-RSPH4A抗体
参阅全部 RSPH4A 一抗 -
描述
兔多克隆抗体to RSPH4A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to amino acids 22-94 (RPWEGKTAAS PQYSEPESSE PLEAKQGPET GRQSRSSRPW SPQSRAKTPL GGPAGPETSS PAPVSPREPS SSP) of Human RSPH4A.
-
阳性对照
- Human bronchus tissue; RT-4 and U-251 MG cell lysates; Human liver and tonsil lysates; A-431 cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122157于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/250 - 1/500.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
| 说明 |
|---|
|
WB
1/250 - 1/500. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
-
功能
Probable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk which is attached to a subfiber of the outer doublet microtubule, and a bulbous head which is attached to the stalk and appears to interact with the projections from the central pair of microtubules. -
疾病相关
Defects in RSPH4A are the cause of primary ciliary dyskinesia type 11 (CILD11) [MIM:612649]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. -
序列相似性
Belongs to the flagellar radial spoke RSP4/6 family. -
细胞定位
Cytoplasm > cytoskeleton > cilium axoneme. Radial spoke. - Information by UniProt
-
数据库链接
- Entrez Gene: 345895 Human
- Omim: 612647 Human
- SwissProt: Q5TD94 Human
- Unigene: 160380 Human
-
别名
- A230081C05 antibody
- CILD11 antibody
- dJ412I7.1 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-RSPH4A antibody (ab122157)ab122157, at 4 µg/ml, staining RSPH4A (green) in PFA-fixed, Triton-permeabilized Human A-431 cells by Immunofluorescence.
-
Western blot - Anti-RSPH4A antibody (ab122157)All lanes : Anti-RSPH4A antibody (ab122157) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPH4A antibody (ab122157)ab122157, at 1/75, staining RSPH4A in paraffin embedded Human bronchus tissue by Immunohistochemistry.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab122157 尚未被引用在任何文献中。