Anti-RSK4 (phospho S232)抗体[EP1524Y] (ab81290)
![Western blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)](https://www.abcam.cn/ps/products/81/ab81290/Images/RSK4-Primary-antibodies-ab81290-2.jpg "Western blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)")
Key features and details
- Rabbit monoclonal [EP1524Y] to RSK4 (phospho S232)
- Suitable for: WB, IHC-P, ELISA, Dot blot
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-RSK4 (phospho S232)抗体[EP1524Y]
参阅全部 RSK4 一抗 -
描述
兔单克隆抗体[EP1524Y] to RSK4 (phospho S232) -
宿主
Rabbit -
特异性
Due to high sequence homology, this antibody might cross-react with RSK1(Q15418) and RSK3(P51812).
This antibody cross-reactivates with PSK3 (phospho S218). The immunogen used for this product shares 100% homology with RSK1 and RSK2, Cross-reactivity with these two proteins has not been confirmed experimentally.
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经测试应用
适用于: WB, IHC-P, ELISA, Dot blotmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Hela cell lysate IHC-P: human muscle tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1524Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ELISA |
Use at an assay dependent concentration.
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| Dot blot |
1/1000.
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| 说明 |
|---|
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WB
1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ELISA
Use at an assay dependent concentration. |
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Dot blot
1/1000. |
靶标
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功能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Autophosphorylated on Ser-389, as part of the activation process. - Information by UniProt
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数据库链接
- Entrez Gene: 27330 Human
- Entrez Gene: 67071 Mouse
- Omim: 300303 Human
- SwissProt: Q9UK32 Human
- SwissProt: Q7TPS0 Mouse
- Unigene: 234478 Human
- Unigene: 37617 Mouse
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别名
- 90 kDa ribosomal protein S6 kinase 6 antibody
- KS6A6_HUMAN antibody
- p90 RSK 6 antibody
see all
图片
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Western blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)All lanes : Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290) at 1/10000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate untreated
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)](https://www.abcam.cn/ps/products/81/ab81290/Images/RSK4-Primary-antibodies-ab81290-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)ab81290, at 1/50, staining human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![ELISA - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)](https://www.abcam.cn/ps/products/81/ab81290/Images/ab81290-356602-anti-rsk4-phospho-s232-antibody-ep1524y-functional-study.png)
ELISA - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)Direct ELISA antigen dose-response curve using ab81290.
Primary antibody: ab81290 (0-1000 ng/ml).
Antigens:
RSK3 (phospho S218) phopspho peptide
RSK3 non-phopspho peptide
RSK4 (phospho S232) phopspho peptide
RSK4 non-phopspho peptide
BSA-branched PEG 40k (0-100 ng/ml).
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.
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![Dot Blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)](https://www.abcam.cn/ps/products/81/ab81290/Images/ab81290-356603-anti-rsk4-phospho-s232-antibody-ep1524y-dot-blot.jpg)
Dot Blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)Dot blot analysis of RSK4 (pS232) phospho peptide (Lane 1), RSK4 non-phospho peptide (Lane 2), RSK3 (pS218) phospho peptide (Lane 3) and RSK3 non-phospho peptide (Lane 4) labeling RSK4 (phospho S232) with ab81290 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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