重组RSK4抗体[EP1982Y](ab76117)|Abcam中文官网

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Western blot - Anti-RSK4 antibody [EP1982Y] (ab76117)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1982Y] to RSK4
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-RSK4抗体[EP1982Y]
参阅全部 RSK4 一抗
描述

兔单克隆抗体[EP1982Y] to RSK4
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide within Human RSK4 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UK32
阳性对照
- SH-SY5Y cell lysate. Human brain glioma tissue. SH-Sy5y cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EP1982Y
同种型

IgG
研究领域

Alternative Versions
- Anti-RSK4 antibody [EP1982Y] - BSA and Azide free (ab247441)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human RSK4 protein (Active) (ab85296)

The Abpromise guarantee

Abpromise™承诺保证使用ab76117于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/25 - 1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB		1/250 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
ICC/IF		1/100 - 1/1000.
IHC-P		1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

说明
Flow Cyt (Intra) 1/25 - 1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/250 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
ICC/IF 1/100 - 1/1000.
IHC-P 1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

功能

Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
序列相似性

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
翻译后修饰

Autophosphorylated on Ser-389, as part of the activation process.
Target information above from: UniProt accession Q9UK32 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 27330 Human
- Entrez Gene: 67071 Mouse
- Entrez Gene: 317203 Rat
- Omim: 300303 Human
- SwissProt: Q9UK32 Human
- SwissProt: Q7TPS0 Mouse
- Unigene: 234478 Human
- Unigene: 37617 Mouse
别名
- 90 kDa ribosomal protein S6 kinase 6 antibody
- KS6A6_HUMAN antibody
- p90 RSK 6 antibody
- p90-RSK 6 antibody
- p90RSK6 antibody
- pp90RSK4 antibody
- Ribosomal protein S6 kinase 90kDa polypeptide 6 antibody
- Ribosomal protein S6 kinase alpha 6 antibody
- Ribosomal protein S6 kinase alpha-6 antibody
- Ribosomal S6 kinase 4 antibody
- rps6ka6 antibody
- RSK 4 antibody
- RSK-4 antibody
- S6K-alpha-6 antibody
see all

Western blot - Anti-RSK4 antibody [EP1982Y] (ab76117)

Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: RSK4 knockout HAP1 cell lysate (40 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76117 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab76117 was shown to recognize RSK4 when RSK4 knockout samples were used, along with additional cross-reactive bands. Wild-type and RSK4 knockout samples were subjected to SDS-PAGE. Ab76117 and ab8245 (loading control to GAPDH) were diluted at 1/250 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-RSK4 antibody [EP1982Y] (ab76117)

All lanes : Anti-RSK4 antibody [EP1982Y] (ab76117) at 1/1000 dilution (purified)

Lane 1 : SH-SY5Y cell lysate
Lane 2 : Caco-2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-RSK4 antibody [EP1982Y] (ab76117)

Immunofluorescence staining of HEK293 cells with purified ab76117 at a working dilution of 1/1000, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab76117 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 antibody [EP1982Y] (ab76117)

Immunohistochemical staining of paraffin embedded rat kidney with purified ab76117 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 antibody [EP1982Y] (ab76117)

Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab76117 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 antibody [EP1982Y] (ab76117)

Immunohistochemical staining of paraffin embedded human kidney with purified ab76117 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Flow Cytometry (Intracellular) - Anti-RSK4 antibody [EP1982Y] (ab76117)

Overlay histogram showing Neuro-2a cells fixed in 2% PFA and stained with purified ab76117 at a dilution of 1 in 70 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
Western blot - Anti-RSK4 antibody [EP1982Y] (ab76117)

Anti-RSK4 antibody [EP1982Y] (ab76117) at 1/500 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 antibody [EP1982Y] (ab76117)

Unpurified ab76117, at 1/50 dilution, staining RSK4 in paraffin-embedded human brain glioma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-RSK4 antibody [EP1982Y] (ab76117)

Immunofluorescent staining of SH-Sy5y cells using unpurified ab76117 at 1/100 dilution.
Anti-RSK4 antibody [EP1982Y] (ab76117)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

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Language
Datasheet download

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发表研究结果有使用 ab76117？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab76117 被引用在 7 文献中.

Hu C et al. Raltitrexed regulates proliferation and apoptosis of HGC-27 cells by upregulating RSK4. BMC Pharmacol Toxicol 23:65 (2022). PubMed: 36031631
Mei Y et al. Overexpression of RSK4 reverses doxorubicin resistance in human breast cancer cells via PI3K/AKT signalling pathway. J Biochem 167:603-611 (2020). PubMed: 31960922
Liang H et al. MiR-548d-3p Promotes Gastric Cancer by Targeting RSK4. Cancer Manag Res 12:13325-13337 (2020). PubMed: 33380838
Hu C et al. Effect of RSK4 on Biological Characteristics of Gastric Cancer. Cancer Manag Res 12:611-619 (2020). PubMed: 32095082
Huo H et al. RSK4 inhibits breast cancer cell proliferation and invasion in vitro, and is correlated with estrogen receptor upregulation in breast cancer. Oncol Rep 42:2777-2787 (2019). PubMed: 31545499
Li Q et al. Estradiol promotes the progression of ER+ breast cancer through methylation-mediated RSK4 inactivation. Onco Targets Ther 12:5907-5916 (2019). PubMed: 31413588
Sun Y et al. Basic anatomy and tumor biology of the RPS6KA6 gene that encodes the p90 ribosomal S6 kinase-4. Oncogene : (2012). WB ; Human . PubMed: 22614021

客户评价及客户问答

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Question

Has Xreactivity with RSK1, 2, 3 been tested or predicted?

Abcam community

Verified customer

Asked on Jan 24 2013

Answer

Thank you for your inquiry.

I've BLASTed the immunogen against RSK1, RSK2 and there was no homology. There was 43% homology with RSK3, but we would not anticipate cross-reactivity since this is so low. We generally say anything with >85% sequence homology would be expected to cross-react. We haven't specifically tested this antibody for its cross-reactivity with any of these proteins, however.

I hope this information helps. Please contact us with any other questions.

Abcam Scientific Support

回复于 Jan 24 2013

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (7)

客户评价及客户问答