重组Anti-RSK3抗体[S05-5E4] (ab317409)
![Western blot - Anti-RSK3 Antibody [S05-5E4] (ab317409)](https://www.abcam.cn/ps/products/317/ab317409/Images/ab317409-1-anti-rsk3-antibody-s05-5e4-western-blot-chok1-hela-chinese-hamster-human.jpg "Western blot - Anti-RSK3 Antibody [S05-5E4] (ab317409)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [S05-5E4] to RSK3
- Suitable for: WB, IHC-P
- Reacts with: Human, Chinese hamster
概述
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产品名称
Anti-RSK3抗体[S05-5E4]
参阅全部 RSK3 一抗 -
描述
兔单克隆抗体[S05-5E4] to RSK3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, Chinese hamster -
免疫原
Synthetic peptide corresponding to Human RSK3.
Database link: Q15349 -
阳性对照
- WB: CHO-K1 and HeLa cell lysate. IHC-P: Human lung cancer tissue.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
S05-5E4 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317409于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 83 kDa.
|
|
| IHC-P |
1/20 - 1/50.
|
| 说明 |
|---|
|
WB
1/1000 - 1/2000. Predicted molecular weight: 83 kDa. |
|
IHC-P
1/20 - 1/50. |
靶标
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功能
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells. -
组织特异性
Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1.
N-terminal myristoylation results in an activated kinase in the absence of added growth factors. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 6196 Human
- Omim: 601685 Human
- SwissProt: Q15349 Human
- Unigene: 655277 Human
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别名
- 90 kDa ribosomal protein S6 kinase 2 antibody
- HU 2 antibody
- KS6A2_HUMAN antibody
see all
图片
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Western blot - Anti-RSK3 Antibody [S05-5E4] (ab317409)All lanes : Anti-RSK3 Antibody [S05-5E4] (ab317409) at 1/1000 dilution
Lane 1 : CHO-K1 cell lysate
Lane 2 : HeLa cell lysate
Predicted band size: 83 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK3 Antibody [S05-5E4] (ab317409)](https://www.abcam.cn/ps/products/317/ab317409/Images/ab317409-536857-anti-rsk3-antibody-s05-5e4-immunohistochemistry-lung-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK3 Antibody [S05-5E4] (ab317409)Immunohistochemical of RSK3 in human lung cancer tissue using ab317409 at dilution 1/20.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317409 尚未被引用在任何文献中。