重组Anti-RSK1 p90 (phospho T573)抗体[EP2133Y] (ab62324)
![Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)](https://www.abcam.cn/ps/products/62/ab62324/Images/ab62324-257528-anti-rsk1-p90-phospho-t573-antibody-ep2133y-western-blot.jpg "Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573)
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RSK1 p90 (phospho T573)抗体[EP2133Y]
参阅全部 RSK1 p90 一抗 -
描述
兔单克隆抗体[EP2133Y] to RSK1 p90 (phospho T573) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- K562 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2133Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62324于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/500.
|
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| WB |
1/500 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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| 说明 |
|---|
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ICC/IF
1/100 - 1/500. |
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WB
1/500 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
靶标
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功能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Autophosphorylated on Ser-380, as part of the activation process. - Information by UniProt
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数据库链接
- Entrez Gene: 6195 Human
- Omim: 601684 Human
- SwissProt: Q15418 Human
- Unigene: 149957 Human
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别名
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
see all
图片
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Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/2000 dilution (purified)
Lane 1 : Untreated Ramos whole cell lysate
Lane 2 : Ramos treated with Phorbol-12-myristate-13-acetate whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)](https://www.abcam.cn/ps/products/62/ab62324/Images/ab62324-257435-anti-rsk1-p90-phospho-t573-antibody-ep2133y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)Immunofluorescence staining of K562 cells with purified ab62324 at a working dilution of 1/500, counter-stained with DAPI. The cells were treated with 500 nM TPA for 15 minutes. Both the treated and untreated cells are shown. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the right hand panels. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom panels - for negative control 1, purified ab62324 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)](https://www.abcam.cn/ps/products/62/ab62324/Images/ab62324-75622-anti-rsk1-p90-phospho-t573-antibody-ep2133y-western-blot.jpg)
Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/500 dilution (Unpurified)
Lane 1 : K562 cell lysate; untreated
Lane 2 : K562 cell lysate; treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP conjugated, at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Beta Tubulin has been included as a loading control. -
![Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)](https://www.abcam.cn/ps/products/62/ab62324/Images/ab62324-4-benefits-of-recombinant-antibodies.png)
Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab62324 被引用在 5 文献中.
- Scerri J et al. PKC-mediated phosphorylation and activation of the MEK/ERK pathway as a mechanism of acquired trastuzumab resistance in HER2-positive breast cancer. Front Endocrinol (Lausanne) 13:1010092 (2022). PubMed: 36329884
- Lebedev TD et al. Identification of cell type-specific correlations between ERK activity and cell viability upon treatment with ERK1/2 inhibitors. J Biol Chem 298:102226 (2022). PubMed: 35787369
- Chaikuad A et al. A unique inhibitor binding site in ERK1/2 is associated with slow binding kinetics. Nat Chem Biol 10:853-60 (2014). PubMed: 25195011
- Talbot AL et al. Aconitase regulation of erythropoiesis correlates with a novel licensing function in erythropoietin-induced ERK signaling. PLoS One 6:e23850 (2011). WB . PubMed: 21887333
- Burd NA et al. Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men. J Physiol 588:3119-30 (2010). PubMed: 20581041