Anti-RSK1 p90 (phospho S221)抗体(ab10695)
Key features and details
- Rabbit polyclonal to RSK1 p90 (phospho S221)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RSK1 p90 (phospho S221)抗体
参阅全部 RSK1 p90 一抗 -
描述
兔多克隆抗体to RSK1 p90 (phospho S221) -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human RSK1 p90 (phospho S221).
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阳性对照
- WB: A431 cells. IHC-P: Human brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
PBS is Ca2+ and Mg2+ free -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit serum by epitope-specific affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10695于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/10 - 1/100.
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| WB |
1/1000. Predicted molecular weight: 90 kDa.
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| 说明 |
|---|
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IHC-P
1/10 - 1/100. |
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WB
1/1000. Predicted molecular weight: 90 kDa. |
靶标
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功能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Autophosphorylated on Ser-380, as part of the activation process. - Information by UniProt
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数据库链接
- Entrez Gene: 6195 Human
- Entrez Gene: 81771 Rat
- Omim: 601684 Human
- SwissProt: Q15418 Human
- SwissProt: Q63531 Rat
- Unigene: 149957 Human
- Unigene: 34915 Rat
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别名
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK1 p90 (phospho S221) antibody (ab10695)
Paraffin embedded human brain tissue (right) stained for RSK1 using ab10695 at 1/20 dilution in immunohistochemical analysis. Negative control without primary antibody (left).
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Western blot - Anti-RSK1 p90 (phospho S221) antibody (ab10695)Peptide Competition - Lysates prepared from A431 cells left unstimulated (1) or stimulated with 100 ng/mL PMA for 30 minutes at 37oC (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% non-fat dried milk-TBST buffer for one hour at room temperature and incubated with ab10695 for one hour at room temperature in a 1% non-fat dried milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate in 1% non-fat dried milk-TBST buffer, and bands were detected by chemiluminescence. The data show induction of ab10695 signal by PMA treatment and that the peptide corresponding to this site blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Co
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab10695 被引用在 7 文献中.
- Deng L et al. Luteolin, a novel p90 ribosomal S6 kinase inhibitor, suppresses proliferation and migration in leukemia cells. Oncol Lett 13:1370-1378 (2017). WB ; Human . PubMed: 28454264
- Duan RB et al. Two p90 ribosomal S6 kinase isoforms are involved in the regulation of mitotic and meiotic arrest in Artemia. J Biol Chem 289:16006-15 (2014). PubMed: 24755224
- Smadja-Lamère N et al. Insulin Activates RSK (p90 Ribosomal S6 Kinase) to Trigger a New Negative Feedback Loop That Regulates Insulin Signaling for Glucose Metabolism. J Biol Chem 288:31165-76 (2013). WB ; Human . PubMed: 24036112
- Zhao Y et al. Involvement of cyclin K posttranscriptional regulation in the formation of Artemia diapause cysts. PLoS One 7:e32129 (2012). WB ; Other species . PubMed: 22363807
- Tangredi MM et al. The C-terminal Kinase and ERK-binding Domains of Drosophila S6KII (RSK) Are Required for Phosphorylation of the Protein and Modulation of Circadian Behavior. J Biol Chem 287:16748-58 (2012). WB ; Drosophila melanogaster . PubMed: 22447936
- Kroczynska B et al. Regulatory effects of ribosomal S6 kinase 1 (RSK1) in IFN{lambda} signaling. J Biol Chem 286:1147-56 (2011). WB ; Human . PubMed: 21075852
- Chen S & Mackintosh C Differential regulation of NHE1 phosphorylation and glucose uptake by inhibitors of the ERK pathway and p90RSK in 3T3-L1 adipocytes. Cell Signal 21:1984-93 (2009). WB ; Mouse . PubMed: 19765648