Anti-RSK1 p90 抗体 [Y81]

• WB

Lab

Western blot - Anti-RSK1 p90 antibody [Y81] (AB32526)

Western blot : Anti-RSK1 p90 antibody [Y81] ab32526 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 83 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in RPS6KA1 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-RSK1 p90 antibody [Y81] (ab32526) at 1/1000 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human RPS6KA1 knockout MCF7 cell line (ab289311) at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

Calu-3 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa,37 kDa

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• WB

Lab

Western blot - Anti-RSK1 p90 antibody [Y81] (AB32526)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : RSK1 knockout HAP1 cell lysate (20 μg)
Lane 3 : MCF-7 cell lysate (20 μg)
Lane 4 : K562 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab32526 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32526 was shown to recognize RSK1 when RSK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and RSK1 knockout samples were subjected to SDS-PAGE. ab32526 and ab8245 (loading control to GADPH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-RSK1 p90 antibody [Y81] (ab32526)

Predicted band size: 82 kDa

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• WB

Unknown

Western blot - Anti-RSK1 p90 antibody [Y81] (AB32526)

All lanes:

Western blot - Anti-RSK1 p90 antibody [Y81] (ab32526) at 1/1000 dilution

All lanes:

MCF7 cell lysate

Predicted band size: 82 kDa

Observed band size: 90 kDa

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• WB

CiteAb

Western blot - Anti-RSK1 p90 antibody [Y81] (AB32526)

RSK1 p90 western blot using anti-RSK1 p90 antibody [Y81] ab32526. Publication image and figure legend from Lu, W., Jia, D., et al., 2017, Sci Rep, PubMed 28827748.

ab32526 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab32526 please see the product overview.

CSEI regulated the expressions of hematopoietic function related proteins in CHRF and K562 cells. After a 24-h CSEI (0, 0.05, 0.1 mg/ml) treatment, the protein expression levels of P-RSK1p90, ELK1 and c-Myc in CHRF cells (a) and K562 cells (b), full-length blots are presented in Supplementary Fig. S5 a, and the expression levels of GATA-1 and GATA-2 in the terminal mature CHRF cells (c) and differentiated K562 cells (d) were detected by western blot, respectively, full-length blots are presented in Supplementary Fig. S5 (b). The quantitative data of these protein levels were normalized by their GAPDH expressions and expressed as a percentage of the corresponding relative intensity of control. The data are shown as the means ± S.E.M. (n = 6). *p < 0.05, **p < 0.01 and ***p < 0.001 versus 0 mg/ml CSEI-treated cells.

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