重组Anti-Rsk 2 / MAPKAP Kinase 1b抗体[Y83] (ab32133)
![Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)](https://www.abcam.cn/ps/products/32/ab32133/Images/ab32133-269787-anti-rsk-2-mapkap-kinase-1b-antibody-y83-western-blot.jpg "Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y83] to Rsk 2 / MAPKAP Kinase 1b
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Rsk 2 / MAPKAP Kinase 1b抗体[Y83]
参阅全部 Rsk 2 / MAPKAP Kinase 1b 一抗 -
描述
兔单克隆抗体[Y83] to Rsk 2 / MAPKAP Kinase 1b -
宿主
Rabbit -
特异性
The antibody recognises Rsk2. It does not cross react with other S6 kinase family members. -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa whole cell lysate (ab150035) and human lymphoma
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y83 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32133于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
组织特异性
Expressed in many tissues, highest levels in skeletal muscle. -
疾病相关
Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Autophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369. - Information by UniProt
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数据库链接
- Entrez Gene: 6197 Human
- Entrez Gene: 110651 Mouse
- Entrez Gene: 501560 Rat
- Omim: 300075 Human
- SwissProt: P51812 Human
- SwissProt: P18654 Mouse
- Unigene: 445387 Human
- Unigene: 328476 Mouse
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别名
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
see all
图片
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Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32133 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.ab32133 was shown to specifically react with Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32133 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773
andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776
secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)](https://www.abcam.cn/ps/products/32/ab32133/Images/ab32133-43987-anti-rsk-2-mapkap-kinase-1b-antibody-y83-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)ab32133, staining human lymphoma by immunohistochemistry, Paraffin embedded tissue
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)](https://www.abcam.cn/ps/products/32/ab32133/Images/ab32133-43988-anti-rsk-2-mapkap-kinase-1b-antibody-y83-western-blot.jpg)
Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133) at 1/5000 dilution + HeLa cell lysate
Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
![Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)](https://www.abcam.cn/ps/products/32/ab32133/Images/ab32133-4-benefits-of-recombinant-antibodies.png)
Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab32133 被引用在 3 文献中.
- Xian F et al. Overexpression of phosphoprotein enriched in astrocytes 15 reverses the damage induced by propofol in hippocampal neurons. Mol Med Rep 20:1583-1592 (2019). PubMed: 31257496
- Takada I DGCR14 induces Il17a gene expression through the ROR?/BAZ1B/RSKS2 complex. Mol Cell Biol 35:344-55 (2015). WB, ChIP ; Mouse . PubMed: 25368387
- Yu G et al. RSK promotes prostate cancer progression in bone through ING3, CKAP2, and PTK6-mediated cell survival. Mol Cancer Res 13:348-57 (2015). PubMed: 25189355