AB109002

重组Anti-RSF1抗体[EPR3749(2)]

Anti-RSF1 antibody [EPR3749(2)]

RabMAb
Recombinant
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(14 Publications)

Rabbit Recombinant Monoclonal RSF1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications.

查看别名

HBXAP, XAP8, RSF1, Remodeling and spacing factor 1, Rsf-1, HBV pX-associated protein 8, Hepatitis B virus X-associated protein, p325 subunit of RSF chromatin-remodeling complex

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with Purified ab109002 at 1 : 100 (8.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with purified ab109002 at 1/80 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Paraffin-embedded human endometrial adenocarcinoma tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Paraffin-embedded human tonsil tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Paraffin-embedded human colon tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

All lanes:

Western blot - Anti-RSF1 antibody [EPR3749(2)] (ab109002) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Predicted band size: 163 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3749(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, Flow Cyt (Intra), WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "Antigen retrieval is recommended.For unpurified use at 1/250- 1/500. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "For unpurified use at 1/500-1/1000.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/80", "FlowCytIntra-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RSF1 also known as Remodeler of Structure of Chromatin 1 is a member of the ATP-dependent chromatin remodeling factors. It has a molecular mass of around 159 kDa. This protein plays a role in altering chromatin architecture by facilitating nucleosome sliding and positioning which is essential for transcriptional regulation. RSF1 expresses in various human tissues particularly in proliferating cells where it assists in regulating gene expression important for cell cycle progression.

Biological function summary

RSF1 participates in cell division and DNA repair processes functioning as part of the RSF complex. This complex along with other chromatin remodelers alters chromatin structure to allow access to DNA. Such mechanisms are critical for allowing correct transcriptional responses during the cell cycle and in response to DNA damage. By modulating nucleosome positioning RSF1 influences the accessibility of transcription factors and other DNA-binding proteins to chromatin.

Pathways

RSF1 involves in the transcriptional regulation and DNA repair pathways. It interacts with proteins like SNF2h within the RSF complex. This interaction is necessary to execute its roles effectively in chromatin remodeling. The involvement of RSF1 in signal transduction pathways also links to the regulation of cell proliferation and survival demonstrating its importance in maintaining cellular homeostasis.

RSF1 associates with cancer development and progression including ovarian and liver carcinoma. Overexpression or mutations within the RSF1 gene can lead to oncogenic transformation supporting tumor growth and metastasis. RSF1's association with other proteins such as key regulators in cancer pathways like p53 indicates its significant role in tumor biology. Understanding its function can provide insights into targeted therapies for cancers involving aberrant RSF1 activity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Regulatory subunit of the ATP-dependent RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed : 12972596, PubMed : 28801535). Binds to core histones together with SMARCA5, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex (PubMed : 12972596). Directly stimulates the ATPase activity of SMARCA1 and SMARCA5 in the RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, respectively (PubMed : 28801535). The RSF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the RSF-5 ISWI chromatin-remodeling complex (PubMed : 28801535). The complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed : 28801535). Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain (PubMed : 11788598, PubMed : 11944984).

See full target information RSF1

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40862741

2025

Chromatin Remodeler RSF1 as an Oncogenic Driver and Therapeutic Target in Esophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Du,Zhili Jia,Yao Lin,Xudong Zhao,Gengsheng Cao,Hengbin Wang

MedComm 6:e70205 PubMed40636284

2025

Depletion of Fat Mass and Obesity-Associated Protein (FTO) Drives Heterochromatin Loss via Lysine Acetyltransferase 8 (KAT8)-Mediated Remodeling and Spacing Factor 1 (RSF1) Acetylation in Skin Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Wang,Lei Zhou,Yun Zhong,Yisheng Cai,Xin Meng,Mengting Chen,Rui Mao,Xin Xiao,Caitan Yi,Yi Guo,Hongfu Xie,Yiya Zhang,Ji Li

Cells 14: PubMed40558555

2025

MicroRNA miR-193b-3p Regulates Esophageal Cancer Progression Through Targeting RSF1.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Lin,Xudong Zhao,Zhenhua Du,Zhili Jia,Siyu Zhou,Gengsheng Cao,Hengbin Wang

Frontiers in cell and developmental biology 11:1168643 PubMed37529237

2023

Recognition of H2AK119ub plays an important role in RSF1-regulated early development.

Applications

Unspecified application

Species

Unspecified reactive species

Saeid Mohammad Parast,Deli Yu,Chunxu Chen,Amanda J Dickinson,Chenbei Chang,Hengbin Wang

Nucleic acids research 49:e64 PubMed33693861

2021

aniFOUND: analysing the associated proteome and genomic landscape of the repaired nascent non-replicative chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Georgios C Stefos,Eszter Szantai,Dimitris Konstantopoulos,Martina Samiotaki,Maria Fousteri

Cancer management and research 12:11845-11855 PubMed33244266

2020

LncRNA NEAT1 Promotes the Progression of Gastric Cancer Through Modifying the miR-1224-5p/RSF1 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Luoluo Yang,Min Wang,Ping He

Cancer biotherapy & radiopharmaceuticals 36:202-210 PubMed33156701

2020

lncRNA-PRLB Confers Paclitaxel Resistance of Ovarian Cancer Cells by Regulating RSF1/NF-κB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzi Zhao,Li Hong

Acta oncologica (Stockholm, Sweden) 59:268-273 PubMed31687881

2019

High RSF1 protein expression is an independent prognostic feature in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Doris Höflmayer,Moslim Hamuda,Cornelia Schroeder,Claudia Hube-Magg,Ronald Simon,Cosima Göbel,Andrea Hinsch,Sören Weidemann,Katharina Möller,Jacob R Izbicki,Frank Jacobsen,Tim Mandelkow,Niclas C Blessin,Florian Lutz,Florian Viehweger,Guido Sauter,Eike Burandt,Patrick Lebok,Maximillian Lennartz,Christoph Fraune,Sarah Minner,Sarah Bonk,Hartwig Huland,Markus Graefen,Thorsten Schlomm,Franziska Büscheck

Nature communications 9:3848 PubMed30242288

2018

The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation.

Applications

Unspecified application

Species

Unspecified reactive species

Ho-Soo Lee,Zhonghui Lin,Sunyoung Chae,Young-Suk Yoo,Byung-Gyu Kim,Youngsoo Lee,Jared L Johnson,You-Sun Kim,Lewis C Cantley,Chang-Woo Lee,Hongtao Yu,Hyeseong Cho

Oncology letters 16:4279-4284 PubMed30214561

2018

Effects of RSF-1 on proliferation and apoptosis of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhui Liu,Junda Gai,Lin Fu,Xiuwei Zhang,Enhua Wang,Qingchang Li

View all publications

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重组Anti-RSF1抗体[EPR3749(2)]