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AB185225

重组Anti-RRP7A抗体[EPR14412] - C-terminal

Anti-RRP7A antibody [EPR14412] - C-terminal

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(1 Publication)

Rabbit Recombinant Monoclonal RRP7A antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

CGI-96, RRP7A, Ribosomal RNA-processing protein 7 homolog A, Gastric cancer antigen Zg14

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RRP7A antibody [EPR14412] - C-terminal (AB185225)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RRP7A antibody [EPR14412] - C-terminal (AB185225)

Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling RRP7A with ab185225 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (AB185225)
  • WB

Supplier Data

Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (AB185225)

All lanes:

Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225) at 1/1000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 32 kDa

false

Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (AB185225)
  • WB

Supplier Data

Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (AB185225)

All lanes:

Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225) at 1/5000 dilution

Lane 1:

MCF7 cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 32 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-RRP7A antibody [EPR14412] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14412

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Primary Antibodies

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Anti-p19 INK4d antibody

Immunocytochemistry/ Immunofluorescence - Anti-p19 INK4d antibody (AB262871)

  • 0
  • 0
Primary Antibodies

AB272580

Anti-ZFAND5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFAND5 antibody (AB272580)

  • 0
  • 0
Primary Antibodies

AB248203

Anti-5HT7 Receptor antibody [EPR6271] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-5HT7 Receptor antibody [EPR6271] - BSA and Azide free (AB248203)

  • 0
  • 0
Primary Antibodies

AB66047

Anti-Serotonin antibody

Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody (AB66047)

  • 5
  • 4
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Conditional Ambient
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RRP7A also known as Ribosomal RNA Processing 7 Homolog A is a protein involved in the assembly of ribosomal subunits. It has a molecular mass of approximately 33 kDa. RRP7A localizes mainly in the nucleolus where ribosomal RNA (rRNA) synthesis and ribosome assembly occur. It exists in various organisms indicating its conserved function across species. The protein is encoded by the RRP7A gene and its expression is observed in many tissues reflecting its essential function in the basic cellular process of ribosome production.
Biological function summary

RRP7A plays an important role in ribosome biogenesis an essential process for the synthesis of proteins. It is part of the small-subunit (SSU) processome complex which is involved in the maturation of 18S rRNA a critical component of the small ribosomal subunit. The SSU processome is essential for early steps of 40S ribosomal subunit assembly. RRP7A acts in association with other proteins within this complex to facilitate the accurate processing of pre-rRNA into functional rRNA molecules necessary for proper ribosomal function.

Pathways

Ribosomal RNA processing represents a critical aspect of translation and protein synthesis. RRP7A fits into the ribosome biogenesis pathway ensuring the correct formation and function of ribosomes. This pathway involves several other proteins including FBL (Fibrillarin) and DDX5 which work alongside RRP7A within the SSU processome. Together these components contribute to the intricate steps of rRNA processing from transcription to the assembly and transport of ribosomal subunits. This makes RRP7A integral to maintaining cellular protein synthesis capacity.

Impaired function of RRP7A can be related to ribosomopathies and certain cancer types due to disruptions in ribosome production. In some cancers altered ribosome biogenesis results in increased cellular proliferation. RRP7A disruptions link to proteins such as MYC a well-known oncogene that can promote ribosome biogenesis when overexpressed. Understanding RRP7A's role in these conditions can inform studies exploring the therapeutic potential of targeting ribosome biogenesis pathways in disease treatment.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Nucleolar protein that is involved in ribosomal RNA (rRNA) processing (PubMed : 33199730). Also plays a role in primary cilia resorption, and cell cycle progression in neurogenesis and neocortex development (PubMed : 33199730). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information RRP7A
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5853 PubMed37730811

2023

Genome-wide probing of eukaryotic nascent RNA structure elucidates cotranscriptional folding and its antimutagenic effect.

Applications

Unspecified application

Species

Unspecified reactive species

Gongwang Yu,Yao Liu,Zizhang Li,Shuyun Deng,Zhuoxing Wu,Xiaoyu Zhang,Wenbo Chen,Junnan Yang,Xiaoshu Chen,Jian-Rong Yang
View all publications
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