Anti-RRN3抗体(ab112052)
Key features and details
- Rabbit polyclonal to RRN3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RRN3抗体
参阅全部 RRN3 一抗 -
描述
兔多克隆抗体to RRN3 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region between amino acids 601-651 of Human RRN3 (NP_060897.3 (GeneID 54700)).
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阳性对照
- HeLa and Jurkat whole cell lysate (ab7899)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112052于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 74 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction. -
序列相似性
Belongs to the RRN3 family. -
翻译后修饰
Phosphorylation is required for participation in rDNA transcription. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 54700 Human
- Entrez Gene: 100173945 Orangutan
- Omim: 605121 Human
- SwissProt: Q9NYV6 Human
- SwissProt: Q5R4N9 Orangutan
- Unigene: 460078 Human
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别名
- AL023001 antibody
- DKFZp566E104 antibody
- E130302O19Rik antibody
see all
图片
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Western blot - Anti-RRN3 antibody (ab112052)All lanes : Anti-RRN3 antibody (ab112052) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-RRN3 antibody (ab112052)ab112052 at 1 µg/ml staining RRN3 in HeLa whole cell lysate immunoprecipitated using ab112052 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: controle IgG. Predicted band size : 74 kDa Exposure time: 10 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab112052 被引用在 7 文献中.
- Chen HF et al. Inhibition of TAF1B impairs ribosome biosynthesis and suppresses cell proliferation in stomach adenocarcinoma through promoting c-MYC mRNA degradation. Heliyon 10:e23167 (2024). PubMed: 38169774
- Peng Y et al. SUMOylation down-regulates rDNA transcription by repressing expression of upstream-binding factor and proto-oncogene c-Myc. J Biol Chem 294:19155-19166 (2019). PubMed: 31694914
- Wei T et al. Small-Molecule Targeting of RNA Polymerase I Activates a Conserved Transcription Elongation Checkpoint. Cell Rep 23:404-414 (2018). WB . PubMed: 29642000
- Von Walden F et al. Muscle contractures in patients with cerebral palsy and acquired brain injury are associated with extracellular matrix expansion, pro-inflammatory gene expression, and reduced rRNA synthesis. Muscle Nerve 58:277-285 (2018). PubMed: 29572878
- Ueshima S et al. Internal Associations of the Acidic Region of Upstream Binding Factor Control Its Nucleolar Localization. Mol Cell Biol 37:N/A (2017). PubMed: 28874518
- Tanaka Y et al. Mild Glucose Starvation Induces KDM2A-Mediated H3K36me2 Demethylation through AMPK To Reduce rRNA Transcription and Cell Proliferation. Mol Cell Biol 35:4170-84 (2015). ChIP ; Human . PubMed: 26416883
- Ueshima S et al. Upstream binding factor-dependent and pre-rRNA transcription-independent association of pre-rRNA processing factors with rRNA gene. Biochem Biophys Res Commun 443:22-7 (2014). PubMed: 24269811