Anti-RRM2抗体[1E1] (ab57653)
![Western blot - Anti-RRM2 antibody [1E1] (ab57653)](https://www.abcam.cn/ps/products/57/ab57653/Images/ab57653-297159-ab57653.jpg "Western blot - Anti-RRM2 antibody [1E1] (ab57653)")
Key features and details
- Mouse monoclonal [1E1] to RRM2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-RRM2抗体[1E1]
参阅全部 RRM2 一抗 -
描述
小鼠单克隆抗体[1E1] to RRM2 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to amino acids 1-111 of Human RRM2
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常规说明
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1E1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab57653于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. -
通路
Genetic information processing; DNA replication. -
序列相似性
Belongs to the ribonucleoside diphosphate reductase small chain family. -
翻译后修饰
Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 6241 Human
- Omim: 180390 Human
- SwissProt: P31350 Human
- Unigene: 226390 Human
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别名
- cb111 antibody
- chunp6884 antibody
- etID309896.20 antibody
see all
图片
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Western blot - Anti-RRM2 antibody [1E1] (ab57653)Anti-RRM2 antibody [1E1] (ab57653) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 45 kDaThis image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (ab57653)](https://www.abcam.cn/ps/products/57/ab57653/Images/ab57653-297157-ab57653icc.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (ab57653)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RRM2 (green) using ab57653 at 10 µg/ml in ICC/IF.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [1E1] (ab57653)](https://www.abcam.cn/ps/products/57/ab57653/Images/ab57653_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [1E1] (ab57653)RRM2 antibody (ab57653) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.
This image was generated using the ascites version of the product.
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![Western blot - Anti-RRM2 antibody [1E1] (ab57653)](https://www.abcam.cn/ps/products/57/ab57653/Images/ab57653_1.jpg)
Western blot - Anti-RRM2 antibody [1E1] (ab57653)RRM2 antibody (ab57653) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (ab57653)](https://www.abcam.cn/ps/products/57/ab57653/Images/RRM2-Primary-antibodies-ab57653-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (ab57653)ICC/IF image of ab57653 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57653, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product. -
![Flow Cytometry - Anti-RRM2 antibody [1E1] (ab57653)](https://www.abcam.cn/ps/products/57/ab57653/Images/RRM2-Primary-antibodies-ab57653-2.jpg)
Flow Cytometry - Anti-RRM2 antibody [1E1] (ab57653)Overlay histogram showing HepG2 cells stained with ab57653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57653, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (38)
ab57653 被引用在 38 文献中.
- Song X et al. Construction of a Novel Ferroptosis-Related Gene Signature for Predicting Survival of Patients With Lung Adenocarcinoma. Front Oncol 12:810526 (2022). PubMed: 35311093
- Liu Y et al. MiR-200c-3p and miR-485-5p overexpression elevates cisplatin sensitivity and suppresses the malignant phenotypes of non-small cell lung cancer cells through targeting RRM2. Thorac Cancer 13:1974-1985 (2022). PubMed: 35599447
- Fu K et al. LncRNA HOTAIR facilitates proliferation and represses apoptosis of retinoblastoma cells through the miR-20b-5p/RRM2/PI3K/AKT axis. Orphanet J Rare Dis 17:119 (2022). PubMed: 35248107
- Cao X et al. MiR-202-3p inhibits the proliferation and metastasis of lung adenocarcinoma cells by targeting RRM2. Ann Transl Med 10:1374 (2022). PubMed: 36660663
- Bothou C et al. Novel Insights into the Molecular Regulation of Ribonucleotide Reductase in Adrenocortical Carcinoma Treatment. Cancers (Basel) 13:N/A (2021). PubMed: 34439352
