重组Anti-RRAD抗体[EPR12856] (ab177151)
![Western blot - Anti-RRAD antibody [EPR12856] (ab177151)](https://www.abcam.cn/ps/products/177/ab177151/Images/ab177151-344800-Anti-rrad-antibody-EPR12856-westernblot-lung-human.jpg "Western blot - Anti-RRAD antibody [EPR12856] (ab177151)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12856] to RRAD
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RRAD抗体[EPR12856]
参阅全部 RRAD 一抗 -
描述
兔单克隆抗体[EPR12856] to RRAD -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human RRAD aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P55042 -
阳性对照
- WB: Human fetal heart, fetal lung and A673 lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12856 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177151于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 33 kDa. |
应用说明
Is unsuitable for IP.
靶标
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功能
May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D. -
组织特异性
Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans. -
序列相似性
Belongs to the small GTPase superfamily. RGK family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6236 Human
- Omim: 179503 Human
- SwissProt: P55042 Human
- Unigene: 1027 Human
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别名
- GTP binding protein RAD antibody
- GTP-binding protein RAD antibody
- RAD antibody
see all
图片
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Western blot - Anti-RRAD antibody [EPR12856] (ab177151)Anti-RRAD antibody [EPR12856] (ab177151) at 1/5000 dilution (Purified) + Human fetal lung tissue lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa -
![Western blot - Anti-RRAD antibody [EPR12856] (ab177151)](https://www.abcam.cn/ps/products/177/ab177151/Images/ab177151-344801-Anti-rrad-antibody-EPR12856-westernblot-a673-human.jpg)
Western blot - Anti-RRAD antibody [EPR12856] (ab177151)Anti-RRAD antibody [EPR12856] (ab177151) at 1/5000 dilution (Purified) + A-673 (Human muscle Ewing's Sarcoma) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDaWe are unsure how to define the extra bands.
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![Western blot - Anti-RRAD antibody [EPR12856] (ab177151)](https://www.abcam.cn/ps/products/177/ab177151/Images/ab177151-205138-anti-rrad-antibody-epr12856-western-blot.jpg)
Western blot - Anti-RRAD antibody [EPR12856] (ab177151)All lanes : Anti-RRAD antibody [EPR12856] (ab177151) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal lung lysate
Lane 3 : A673 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa -
![Anti-RRAD antibody [EPR12856] (ab177151)](https://www.abcam.cn/ps/products/177/ab177151/Images/ab177151-5-benefits-of-recombinant-antibodies.png)
Anti-RRAD antibody [EPR12856] (ab177151)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab177151 尚未被引用在任何文献中。