AB177151

重组Anti-RRAD抗体[EPR12856]

Anti-RRAD antibody [EPR12856]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal RRAD antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

查看别名

RAD, RRAD, GTP-binding protein RAD, RAD1, Ras associated with diabetes

3 Images

Lab

Western blot - Anti-RRAD antibody [EPR12856] (AB177151)

We are unsure how to define the extra bands.

All lanes:

Western blot - Anti-RRAD antibody [EPR12856] (ab177151) at 1/5000 dilution

All lanes:

A-673 (Human muscle Ewing's Sarcoma) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

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Supplier Data

Western blot - Anti-RRAD antibody [EPR12856] (AB177151)

All lanes:

Western blot - Anti-RRAD antibody [EPR12856] (ab177151) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal lung lysate at 10 µg

Lane 3:

A673 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

false

Lab

Western blot - Anti-RRAD antibody [EPR12856] (AB177151)

All lanes:

Western blot - Anti-RRAD antibody [EPR12856] (ab177151) at 1/5000 dilution

All lanes:

Human fetal lung tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-RRAD antibody [EPR12856] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12856

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RRAD also known as Ras-related associated with diabetes is a small GTPase belonging to the Ras superfamily. It weighs approximately 29 kDa and exhibits expression in cardiac and skeletal muscle tissues as well as the nervous system. This protein functions mechanically by binding and hydrolyzing GTP a process critical for its role in intracellular signaling. RRAD is known for its ability to affect various molecular interactions through its GTPase activity.

Biological function summary

RRAD regulates glucose transport and metabolism influencing energy balance in cells. It does not integrate into a larger protein complex but instead acts on its own to modify glucose transporter activity. RRAD impacts how cells take up and use glucose which is significant for maintaining cellular energy requirements and contributes to processes that control cell growth and survival.

Pathways

Understanding the signaling mechanisms of RRAD involves examining the insulin signaling pathway and the glucose metabolism pathway. RRAD impacts insulin receptor signaling and modulation of glucose transporter type 4 (GLUT4) activity. RRAD holds a relationship with other proteins such as AKT and PI3K in these pathways which play critical roles in transmitting signals that manage glucose uptake and regulation.

The alteration in RRAD expression or function associates with diabetes and cardiovascular diseases. In diabetes abnormal RRAD function affects insulin signaling contributing to insulin resistance. In cardiovascular disease RRAD dysregulation can influence cardiac function through its impact on energy metabolism. The protein interacts with others like GLUT4 and AKT which are relevant in these disease contexts implicating RRAD in pathways that influence disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May regulate basal voltage-dependent L-type Ca(2+) currents and be required for beta-adrenergic augmentation of Ca(2+) influx in cardiomyocytes, thereby regulating increases in heart rate and contractile force (By similarity). May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents (By similarity). Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway (PubMed : 18056528). Inhibits phosphorylation and activation of CAMK2D (PubMed : 18056528).

See full target information RRAD

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8712 PubMed41027885

2025

RNA-mediated condensation of TFE3 oncofusions facilitates transcriptional hub formation to promote translocation renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Guo,Rongjie Zhao,Yi-Tsang Lee,Junhua Huang,James Wengler,Logan Rivera,Tingting Hong,Tianlu Wang,Kunjal Rathod,Ashley Suris,Yitian Wu,Xiaoli Cai,Rui Wang,Yubin Zhou,Yun Huang

Cell death & disease 15:376 PubMed38811531

2024

The enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Aoming Cheng,Qiaoshi Xu,Bo Li,Lirui Zhang,Hao Wang,Chang Liu,Zhengxue Han,Zhien Feng

View all publications

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重组Anti-RRAD抗体[EPR12856]