重组Anti-RPUSD3抗体[EPR12911] (ab181121)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12911] to RPUSD3
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RPUSD3抗体[EPR12911]
参阅全部 RPUSD3 一抗 -
描述
兔单克隆抗体[EPR12911] to RPUSD3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- U87-MG, SH-SY5Y, 293T or Human fetal brain lysate, SH-SY5Y or U87-MG cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12911 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181121于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/290.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/2000. Predicted molecular weight: 38 kDa.
|
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ICC/IF |
1/100 - 1/250.
|
说明 |
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Flow Cyt (Intra)
1/290. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Predicted molecular weight: 38 kDa. |
ICC/IF
1/100 - 1/250. |
靶标
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相关性
RPUSD3 belongs to the pseudouridine synthase rluA family. Members of this family are involved in modifying bases in RNA molecules. Pseudouridine synthases are responsible for synthesis of pseudouridine from uracil. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 285367 Human
- Entrez Gene: 101122 Mouse
- Entrez Gene: 362416 Rat
- SwissProt: Q6P087 Human
- SwissProt: Q14AI6 Mouse
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别名
- FLJ34707 antibody
- RNA pseudouridylate synthase domain containing 3 antibody
- RPUSD 3 antibody
图片
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Intracellular Flow Cytometry analysis of U87-MGcells using ab181121at a 1/290 dilution (red) or aRabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary usedat a 1/150 dilution.
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Immunofluorescence analysis of SH-SY5Y cells (fixative 4% paraformaldehyde) labeling RPUSD3 with ab181121 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
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All lanes : Anti-RPUSD3 antibody [EPR12911] (ab181121) at 1/1000 dilution
Lane 1 : U87-MG lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : 293T lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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