AB201454

重组Anti-RPS8抗体[EPR17334]

Anti-RPS8 antibody [EPR17334]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal RPS8 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

查看别名

OK/SW-cl.83, RPS8, Small ribosomal subunit protein eS8, 40S ribosomal protein S8

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] (AB201454)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] (AB201454)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton-X100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling RPS8 with ab201454 at 1/600 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is stained with ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution (red).
-ve control 1 : ab201454 at 1/600 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution.
-ve control 2 : ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution.

Flow Cytometry (Intracellular) - Anti-RPS8 antibody [EPR17334] (AB201454)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RPS8 antibody [EPR17334] (AB201454)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed A549 (Human lung carcinoma) cells labeling RPS8 with ab201454 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] (AB201454)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton-X100 permeabilized A549 (Human lung carcinoma) cells labeling RPS8 with ab201454 at 1/600 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution (green). Confocal image showing cytoplasmic staining on A549 cell line. The nuclear counter stain is DAPI (blue). Tubulin is stained with ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution (red).
-ve control 1 : ab201454 at 1/600 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution.
-ve control 2 : ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution.

Supplier Data

Immunoprecipitation - Anti-RPS8 antibody [EPR17334] (AB201454)

RPS8 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab201454 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab201454 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

Lane 1 : HEK293 whole cell lysate 10 μg (Input). Lane 2 : ab201454 IP in HEK293 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab201454 in HEK293 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-RPS8 antibody [EPR17334] (ab201454)

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-RPS8 antibody [EPR17334] (AB201454)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS8 antibody [EPR17334] (ab201454) at 1/10000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

Lane 3:

A549 (Human lung carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-RPS8 antibody [EPR17334] (AB201454)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS8 antibody [EPR17334] (ab201454) at 1/10000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human pancreas lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-RPS8 antibody [EPR17334] (AB201454)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS8 antibody [EPR17334] (ab201454) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 10s

不同偶联物与剂型 (1)

Carrier free

Anti-RPS8 antibody [EPR17334] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17334

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IP, ICC/IF, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/600", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/250", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Ribosomal protein S8 (RPS8) also known as S8 is a component of the small 40S subunit of the eukaryotic ribosome. This protein has a mass of approximately 20 kDa and is widely expressed in most tissues reflecting its fundamental role in cellular processes. RPS8 belongs to the ribosomal protein S8P family and plays a mechanical role in the assembly and function of the ribosome where it contributes to protein synthesis by facilitating mRNA translation.

Biological function summary

Ribosomal protein S8 is fundamental in translation an essential cellular process that translates mRNA sequences into polypeptide chains. As part of the ribosomal 40S subunit RPS8 aids in the initiation of translation by interacting with other ribosomal proteins and rRNA to form a functional ribosome. This subunit along with the 60S large subunit comprises the active ribosome complex engaged in translating genetic information into the proteome.

Pathways

RPS8 is involved in the protein synthesis pathway a critical driver of cellular proliferation and metabolism. It participates actively in the mRNA surveillance pathway ensuring the efficiency and accuracy of translation. It interacts with other proteins such as RPS6 and RPS14 which are also constituents of the small ribosomal subunit acting synergistically to regulate the efficacy of the translation process. Moreover RPS8 involvement in the ribosome biogenesis pathway emphasizes its integral role in cellular growth and division.

Alterations in ribosomal protein S8 are connected to ribosomopathies a group of disorders characterized by defective ribosome function. Dysregulation or mutations in RPS8 can lead to Diamond-Blackfan anemia a rare congenital disorder causing impaired erythropoiesis. Additionally RPS8 has correlations with cancer where aberrant expression levels contribute to tumorigenesis. In cancer RPS8 might interact with oncogenic proteins such as MYC heightening the proliferative potential of cancer cells.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Component of the small ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS8

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:438-448 PubMed39984653

2025

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties.

Applications

Unspecified application

Species

Unspecified reactive species

Dennis K Jeppesen,Zachary C Sanchez,Noah M Kelley,James B Hayes,Jessica Ambroise,Emma N Koory,Evan Krystofiak,Nilay Taneja,Qin Zhang,Matthew M Dungan,Olivia L Perkins,Matthew J Tyska,Ela W Knapik,Kevin M Dean,Amanda C Doran,Robert J Coffey,Dylan T Burnette

The Journal of biological chemistry 300:107958 PubMed39510176

2024

STAG2 promotes naive-primed transition via activating Lin28a transcription in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Chen,Mingkang Jia,Gan Zhao,Yumin Liu,Yihong Song,Mengjie Sun,Wangfei Chi,Xiangyang Wang,Qing Jiang,Guangwei Xin,Chuanmao Zhang

Science advances 10:eadn2050 PubMed38809982

2024

CLUH maintains functional mitochondria and translation in motoneuronal axons and prevents peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Zaninello,Tim Schlegel,Hendrik Nolte,Mujeeb Pirzada,Elisa Savino,Esther Barth,Ines Klein,Hauke Wüstenberg,Tesmin Uddin,Lisa Wolff,Brunhilde Wirth,Helmar C Lehmann,Jean-Michel Cioni,Thomas Langer,Elena I Rugarli

Molecular cell 81:2808-2822.e10 PubMed34111399

2021

Translation stress and collided ribosomes are co-activators of cGAS.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wan,Szymon Juszkiewicz,Daniel Blears,Prashanth Kumar Bajpe,Zhong Han,Peter Faull,Richard Mitter,Aengus Stewart,Ambrosius P Snijders,Ramanujan S Hegde,Jesper Q Svejstrup

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-RPS8抗体[EPR17334]