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重组RabMAb

重组Anti-RPS6 (phospho S235)抗体[EP1338(2)Y] - Ribosome Marker (ab80158)

Submit a review Submit a question References (3)
Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
  • Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
  • Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
  • Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
  • Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1338(2)Y] to RPS6 (phospho S235) - Ribosome Marker
  • Suitable for: WB, IP, IHC-P, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-RPS6 (phospho S235)抗体[EP1338(2)Y] - Ribosome Marker
    参阅全部 RPS6 一抗

  • 描述

    兔单克隆抗体[EP1338(2)Y] to RPS6 (phospho S235) - Ribosome Marker

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-P, Dot blotmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa cell lysate, untreated or treated with Calyculin A. IHC: Human breast adenocarcinoma tissue. IP: 293T lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EP1338(2)Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab80158于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/10000 - 1/200000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP
1/30.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Dot blot
Use at an assay dependent concentration.
说明
WB
1/10000 - 1/200000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP
1/30.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Dot blot
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

  • 序列相似性

    Belongs to the ribosomal protein S6e family.

  • 翻译后修饰

    Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 40S ribosomal protein S6 antibody
      • Air8 antibody
      • NP33 antibody
      • Phosphoprotein NP33 antibody
      • Pp30 antibody
      • Ribosomal protein S6 antibody
      • RP S6 antibody
      • rps6 antibody
      • RS6 antibody
      • RS6_HUMAN antibody
      • S6 antibody
      • S6 Ribosomal Protein antibody
      see all

    图片

    • Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
      Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
      All lanes : Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) at 1/1000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h. Whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h, then 20% FBS incubated for 90 minutes. Whole cell lysates
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h, then 20% FBS incubated for 90 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 30 kDa
      Observed band size: 30 kDa


      Exposure time: 1 minute


      Blocking and diluting  buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)

      ab80158 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
      Immunoprecipitation - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)

      RPS6 was immunoprecipitated from 0.35 mg 293T (Human embryonic kidney epithelial cell) treated with Calyculin A(100nM 30min) whole cell lysate 10 µg with ab80158 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: 293T (Human embryonic kidney epithelial cell) treated with Calyculin A(100nM 30min) whole cell lysate 10 µg

      Lane 2: ab80158 IP in 293T treated with Calyculin A(100nM 30min) whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab80158 in 293T treated with Calyculin A(100nM 30min) whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

       

    • Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
      Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)

      Dot Blot analysis of -

      Lane 1: Rb (pS235) phospho peptide

      Lane 2: Rb (pS236) phospho peptide

      Lane 3: Rb (pS235 & pS236) phospho peptide

      Lane 4: Rb non-phospho peptide

      5% NFDM/TBST was used as the diluting and blocking buffer.

      ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

    • Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
      Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
      All lanes : Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) at 1/200000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 30 kDa
      Observed band size: 30 kDa

    • Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
      Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)

    文献 (3)

    发表研究结果有使用 ab80158？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab80158 被引用在 3 文献中.

    • Tian K  et al. AKR1B10 inhibits the proliferation and metastasis of hepatocellular carcinoma cells by regulating the PI3K/AKT pathway. Oncol Lett 27:18 (2024). PubMed: 38034486
    • Li Q  et al. Heterogeneity of cell composition and origin identified by single-cell transcriptomics in renal cysts of patients with autosomal dominant polycystic kidney disease. Theranostics 11:10064-10073 (2021). PubMed: 34815804
    • Chuang JH  et al. Differentiation of glutamatergic neurons from mouse embryonic stem cells requires raptor S6K signaling. Stem Cell Res 11:1117-1128 (2013). Mouse . PubMed: 23988668

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