Anti-RPS6抗体- Ribosome Marker (ab70227)
Key features and details
- Rabbit polyclonal to RPS6 - Ribosome Marker
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RPS6抗体- Ribosome Marker
参阅全部 RPS6 一抗 -
描述
兔多克隆抗体to RPS6 - Ribosome Marker -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Reptile, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residues 100 and 150 of human RPS6. ( NP_001001.2).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70227于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. -
序列相似性
Belongs to the ribosomal protein S6e family. -
翻译后修饰
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. - Information by UniProt
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数据库链接
- Entrez Gene: 396148 Chicken
- Entrez Gene: 787914 Cow
- Entrez Gene: 474722 Dog
- Entrez Gene: 6194 Human
- Entrez Gene: 20104 Mouse
- Entrez Gene: 667739 Mouse
- Entrez Gene: 100038023 Pig
- Entrez Gene: 100338367 Rabbit
see all -
别名
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
see all
图片
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Western blot - Anti-RPS6 antibody - Ribosome Marker (ab70227)This image is courtesy of an anonymous AbreviewAnti-RPS6 antibody - Ribosome Marker (ab70227) at 1/1000 dilution + Caenorhabditis elegans whole adult tissue lysate at 200 µg
Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa (possible non-specific binding), 26 kDa (possible non-specific binding), 40 kDa (possible non-specific binding)
Exposure time: 40 seconds
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Western blot - Anti-RPS6 antibody - Ribosome Marker (ab70227)All lanes : Anti-RPS6 antibody - Ribosome Marker (ab70227) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-RPS6 antibody - Ribosome Marker (ab70227)Detection of Human RPS6 by Immunoprecipitation. Samples: A) Whole cell lysate from HeLa cells (1 mg/IP; 1/4 of IP loaded) using affinity purified ab70227 at 3 µg/mg lysate for IP and at 0.04 µg/ml for subequent WB detection. Second lane represents IgG IP control.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab70227 被引用在 12 文献中.
- Zhang ZJ et al. EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion. Heliyon 9:e15305 (2023). PubMed: 37101626
- Hattori K et al. The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation. Medicines (Basel) 8:N/A (2021). PubMed: 33535532
- Liu Z et al. SNORA23 inhibits HCC tumorigenesis by impairing the 2'-O-ribose methylation level of 28S rRNA. Cancer Biol Med 19:104-19 (2021). PubMed: 33710804
- Segreto R et al. The TOR kinase pathway is relevant for nitrogen signaling and antagonism of the mycoparasite Trichoderma atroviride. PLoS One 16:e0262180 (2021). PubMed: 34972198
- Matsumoto N et al. PP1C and PP2A are p70S6K Phosphatases Whose Inhibition Ameliorates HLD12-Associated Inhibition of Oligodendroglial Cell Morphological Differentiation. Biomedicines 8:N/A (2020). PubMed: 32316234