Anti-RPS6抗体- Ribosome Marker (ab40820)
Key features and details
- Rabbit polyclonal to RPS6 - Ribosome Marker
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RPS6抗体- Ribosome Marker
参阅全部 RPS6 一抗 -
描述
兔多克隆抗体to RPS6 - Ribosome Marker -
宿主
Rabbit -
特异性
Replenishment batches of our polyclonal antibody, ab40820 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab225676.
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经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe, a wide range of other species -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RPS6.
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阳性对照
- WB: HeLa, Jurkat, A431, HEK293, HepG2, MCF7, and SHSY-5Y whole cell lysates. IHC-P: Human skin melanoma tissue. ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40820于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).
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ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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说明 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. -
序列相似性
Belongs to the ribosomal protein S6e family. -
翻译后修饰
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. - Information by UniProt
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数据库链接
- Entrez Gene: 6194 Human
- Entrez Gene: 20104 Mouse
- Entrez Gene: 667739 Mouse
- Entrez Gene: 29304 Rat
- Omim: 180460 Human
- SwissProt: P62753 Human
- SwissProt: P62754 Mouse
- SwissProt: P62755 Rat
see all -
别名
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
see all
图片
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All lanes : Anti-RPS6 antibody - Ribosome Marker (ab40820) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted? -
Western blot - Anti-RPS6 antibody - Ribosome Marker (ab40820)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.All lanes : Anti-RPS6 antibody - Ribosome Marker (ab40820) at 2 µg/ml
Lane 1 : Whole cell lysate from mouse NIH-3T3 cell line
Lane 2 : Whole cell lysate from mouse MEF cell line
Lane 3 : Whole cell lysate from rat PC12 cell line
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated pig anti rabbit Ig at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 28 kDa
Exposure time: 5 minutes
Detection method: Supersignal West Dura -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody - Ribosome Marker (ab40820)
IHC image of RPS6 staining in a section of formalin-fixed paraffin-embedded normal human skin melanoma* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab40820, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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ab40820 stained in A431 cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40820 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
数据表及文件
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SDS download
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Datasheet download
文献 (47)
ab40820 被引用在 47 文献中.
- Carvajal Ibañez D et al. Interferon regulates neural stem cell function at all ages by orchestrating mTOR and cell cycle. EMBO Mol Med 15:e16434 (2023). PubMed: 36636818
- Liang J et al. Divergence of TORC1-mediated stress response leads to novel acquired stress resistance in a pathogenic yeast. PLoS Pathog 19:e1011748 (2023). PubMed: 37871123
- Li L et al. Salicylic acid fights against Fusarium wilt by inhibiting target of rapamycin signaling pathway in Fusarium oxysporum. J Adv Res 39:1-13 (2022). PubMed: 35777900
- Choudhary K et al. S. cerevisiae Cells Can Grow without the Pds5 Cohesin Subunit. mBio 13:e0142022 (2022). PubMed: 35708277
- McCarthy A et al. Msl3 promotes germline stem cell differentiation in female Drosophila. Development 149:N/A (2022). PubMed: 34878097