Anti-RPS3A抗体- N-terminal (ab226356)
Key features and details
- Rabbit polyclonal to RPS3A - N-terminal
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RPS3A抗体- N-terminal
参阅全部 RPS3A 一抗 -
描述
兔多克隆抗体to RPS3A - N-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
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免疫原
Synthetic peptide within Human RPS3A aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: P61247 -
阳性对照
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human ovarian cancer tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226356 was affinity purified using an epitope specific to RPS3A immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226356于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 30 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
May play a role during erythropoiesis through regulation of transcription factor DDIT3. -
序列相似性
Belongs to the ribosomal protein S3Ae family. -
细胞定位
Cytoplasm. Nucleus. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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数据库链接
- Entrez Gene: 6189 Cow
- Entrez Gene: 6189 Human
- Entrez Gene: 20091 Mouse
- Entrez Gene: 29288 Rat
- Entrez Gene: 444841 Xenopus laevis
- Entrez Gene: 493437 Xenopus tropicalis
- Entrez Gene: 337240 Zebrafish
- Omim: 180478 Human
see all -
别名
- 40S ribosomal protein S3a antibody
- Fte 1 antibody
- Fte-1 antibody
see all
图片
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Western blot - Anti-RPS3A antibody - N-terminal (ab226356)All lanes : Anti-RPS3A antibody - N-terminal (ab226356) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 3 secondsCell lysates were prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-RPS3A antibody - N-terminal (ab226356)RPS3A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226356 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226356 at 0.4 µg/ml.
Lane 1: ab226356 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody - N-terminal (ab226356)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for RPS3A using ab226356 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226356 尚未被引用在任何文献中。