重组Anti-RPS3A抗体[EPR11982(B)]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] (AB171742)

Immunohistochemistry of paraffin-embedded Human pancreas tissue labeling RPS3A using ab171742 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody [EPR11982(B)] (AB171742)

Immunohistochemistry of paraffin-embedded Human brain tissue labeling RPS3A using ab171742 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-RPS3A antibody [EPR11982(B)] (AB171742)

All lanes:

Western blot - Anti-RPS3A antibody [EPR11982(B)] (ab171742) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Predicted band size: 30 kDa

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• WB

CiteAb

Western blot - Anti-RPS3A antibody [EPR11982(B)] (AB171742)

Western Blotting using Anti-RPS3A antibody [EPR11982(B)], ab171742. Publication image from Li, J. et al., 2015, Nucleic Acids Res, 26405197. Legend direct from paper.

Co-translational histone H3K9 methylation. (A) Scheme illustrating the procedure employed to in vitro puromycilate nascent polypeptides. (B) Western blot analysis of the puromycilation reaction utilizing purified ribosomes, under the conditions described. (C–D) Western blot analysis of purified biotin-conjugated puromycilated polypeptides, as indicated.

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• WB

CiteAb

Western blot - Anti-RPS3A antibody [EPR11982(B)] (AB171742)

Western Blotting using Anti-RPS3A antibody [EPR11982(B)], ab171742. Publication image from Li, J. et al., 2015, Nucleic Acids Res, 26405197. Legend direct from paper.

Histone H3K9 mono- and dimethylation occurs at ribosomes. (A) Increasing amounts of ribosomes (0.2 and 0.6 ug of ribosomal RNA) were analyzed by Western blot, as indicated. Input corresponded to 0.5% of the total amount of HeLa derived extract loaded onto the sucrose cushion for the purification of ribosomes, corresponding to 15 ug of ribosomal RNA. (B) Increasing amounts of ribosomes (0.5 and 1.5 ug of ribosomal RNA) were analyzed by agarose gel for the presence of the 28S and 18S ribosomal RNAs. (C) Isotopic distribution of the mono- and dimethylated H3 (9–17) peptides (ion charge = 2+), from which the MS2 spectrum was acquired to locate the methyl groups on K9 (Supplementary Figure S2) (m/z = the mass-to-charge ratio; m = the monoisotopic mass value; δmass = difference between the excepted and the measured masses; R = resolution of the MS measurement). (D) Quantification of the modifications on H3 peptides (3–8) and (18–26) from ribosomal bound histones H3. Standard deviation derived from three independent experiments.

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