重组Anti-RPS3抗体[EPR7808] - Ribosome Marker (ab128995)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7808] to RPS3 - Ribosome Marker
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RPS3抗体[EPR7808] - Ribosome Marker
参阅全部 RPS3 一抗 -
描述
兔单克隆抗体[EPR7808] to RPS3 - Ribosome Marker -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2, PC-12 and Neuro-2a lysate IHC-P: Human kidney tissue ICC/IF: Neuro-2a cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.36 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7808 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)
- APC Anti-RPS3 antibody [EPR7808] – Ribosome Marker (ab319264)
- PE Anti-RPS3 antibody [EPR7808] – Ribosome Marker (ab319412)
- Alexa Fluor® 488 Anti-RPS3 antibody [EPR7808] – Ribosome Marker (ab319546)
- Alexa Fluor® 647 Anti-RPS3 antibody [EPR7808] – Ribosome Marker (ab319659)
- Alexa Fluor® 594 Anti-RPS3 antibody [EPR7808] – Ribosome Marker (ab319763)
- Alexa Fluor® 555 Anti-RPS3 antibody [EPR7808] – Ribosome Marker (ab319905)
- Alexa Fluor® 750 Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab321023)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128995于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
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ICC/IF
1/100. |
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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序列相似性
Belongs to the ribosomal protein S3P family.
Contains 1 KH type-2 domain. -
细胞定位
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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数据库链接
- Entrez Gene: 6188 Human
- Entrez Gene: 27050 Mouse
- Entrez Gene: 140654 Rat
- Omim: 600454 Human
- SwissProt: P23396 Human
- SwissProt: P62908 Mouse
- SwissProt: P62909 Rat
- Unigene: 546286 Human
see all -
别名
- 40S ribosomal protein S3 antibody
- fb13d09 antibody
- FLJ26283 antibody
see all
图片
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All lanes : Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab128995) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa -
Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) labeling RPS3 with ab128995 at 1/100 dilution. ab150077, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Cells were fixed with 100% Methanol. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/00 was used as counterstain. Nuclei were stained blue with DAPI.Confocal image showing cytoplasmic staining in Neuro-2a cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab128995)
ab128995, at a 1/50 dilution, staining RPS3 in Human kidney, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab128995 被引用在 4 文献中.
- Lei XX et al. SOX1 acts as a tumor hypnotist rendering nasopharyngeal carcinoma cells refractory to chemotherapy. Cell Death Discov 9:194 (2023). PubMed: 37369660
- Yang L et al. VPS9D1-AS1 overexpression amplifies intratumoral TGF-β signaling and promotes tumor cell escape from CD8+ T cell killing in colorectal cancer. Elife 11:N/A (2022). PubMed: 36458816
- Singh M et al. Translation and codon usage regulate Argonaute slicer activity to trigger small RNA biogenesis. Nat Commun 12:3492 (2021). PubMed: 34108460
- Matthes F et al. Inhibition of the MID1 protein complex: a novel approach targeting APP protein synthesis. Cell Death Discov 4:4 (2018). PubMed: 29531801