AB128995

重组Anti-RPS3抗体[EPR7808] - Ribosome Marker

Anti-RPS3 antibody [EPR7808] - Ribosome Marker

RabMAb
Recombinant
Lab Essentials
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(11 Publications)

Rabbit Recombinant Monoclonal RPS3 antibody. Ribosome marker. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 11 publications.

查看别名

OK/SW-cl.26, RPS3, Small ribosomal subunit protein uS3, 40S ribosomal protein S3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (AB128995)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (AB128995)

ab128995 at a 1/50 dilution staining RPS3 in Human kidney using Immunohistochemistry Formalin/PFA-fixed paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (AB128995)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (AB128995)

Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) labeling RPS3 with ab128995 at 1/100 dilution. ab150077, Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Cells were fixed with 100% Methanol. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/00 was used as counterstain. Nuclei were stained blue with DAPI.

Confocal image showing cytoplasmic staining in Neuro-2a cells.

Unknown

Western blot - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (AB128995)

All lanes:

Western blot - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab128995) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Lane 3:

Neuro-2a cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 27 kDa

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OI-RD Scanning - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (AB128995)

OI-RD Scanning

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OI-RD Scanning - Anti-RPS3 antibody [EPR7808] - Ribosome Marker (AB128995)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (8)

Carrier free

Anti-RPS3 antibody [EPR7808] - BSA and Azide free
660 APC

APC Anti-RPS3 antibody [EPR7808] – Ribosome Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-RPS3 antibody [EPR7808] – Ribosome Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-RPS3 antibody [EPR7808] – Ribosome Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-RPS3 antibody [EPR7808] – Ribosome Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-RPS3 antibody [EPR7808] – Ribosome Marker
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-RPS3 antibody [EPR7808] - Ribosome Marker
578 PE

PE Anti-RPS3 antibody [EPR7808] – Ribosome Marker

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7808

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RPS3 also known as Ribosomal Protein S3 is an important component of the 40S ribosomal subunit. It has a molecular mass of approximately 26 kDa. Functionally RPS3 plays a role in the translation process as part of the ribosome machinery assisting in the assembly of proteins by decoding messenger RNA. Expression of RPS3 is ubiquitous across various tissues suggesting a fundamental role for the protein in cellular functions.

Biological function summary

Protein synthesis relies heavily on components like RPS3 within the ribosomal complex where it interacts with other ribosomal proteins and rRNA to ensure accurate translation. RPS3 also exhibits DNA repair activities independently of its ribosomal function. It binds to DNA damaged sites adding a layer of protection to help maintain genomic stability by facilitating repair processes.

Pathways

RPS3 participates actively in the mRNA translation and DNA repair pathways. Inside the ribosome biogenesis pathway RPS3 interacts closely with other ribosomal proteins like RPS19 and RPS20 to form functional ribosomal units. In the context of DNA repair it aligns with interaction networks that overlap with the NHEJ (Non-Homologous End Joining) pathway thereby influencing genomic maintenance alongside proteins such as XRCC5.

The dysfunction of RPS3 associates with neurodegenerative diseases and certain types of cancer. Aberrant levels or mutations can disrupt protein synthesis and DNA repair mechanisms potentially leading to tumorigenesis. In the context of cancer RPS3 shows interactions with oncogenes such as MYC linking it to pathway disturbances that can facilitate tumor progression. Similarly its misregulation is noticed in neurodegenerative contexts where protein synthesis and repair imbalances contribute to disease pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the small ribosomal subunit (PubMed : 23636399, PubMed : 8706699). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 8706699). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed : 7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed : 15707971). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed : 14706345). Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed : 18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed : 15518571). Enhances the uracil excision activity of UNG1 (PubMed : 18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed : 18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed : 23911537). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed : 17049931). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed : 18045535). Represses its own translation by binding to its cognate mRNA (PubMed : 20217897). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed : 19656744). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed : 23131551). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed : 14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed : 20605787). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed : 22510408).

See full target information RPS3

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1619538 PubMed40895568

2025

A fusion ORF3a-E subgenomic RNA involved in SARS-CoV-2 infection efficacy by influencing cellular protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Zhang,Jing Li,Xinglong Zhang,Xin Zhang,Jiali Li,Heng Li,Xin Zhao,Zihan Zhang,Yingyan Li,Keqi Chen,Shasha Peng,Haijing Shi,Longding Liu

The EMBO journal 44:801-823 PubMed39690241

2024

A nuclear protein quality control system for elimination of nucleolus-related inclusions.

Applications

Unspecified application

Species

Unspecified reactive species

Lorène Brunello,Jolanta Polanowska,Léo Le Tareau,Chantal Maghames,Virginie Georget,Charlotte Guette,Karima Chaoui,Stéphanie Balor,Marie-Françoise O'Donohue,Marie-Pierre Bousquet,Pierre-Emmanuel Gleizes,Dimitris P Xirodimas

iScience 27:109172 PubMed38414864

2024

Disruption of mitochondrial energy metabolism is a putative pathogenesis of Diamond-Blackfan anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Rudan Xiao,Lijuan Zhang,Zijuan Xin,Junwei Zhu,Qian Zhang,Guangmin Zheng,Siyun Chu,Jing Wu,Lu Zhang,Yang Wan,Xiaojuan Chen,Weiping Yuan,Zhaojun Zhang,Xiaofan Zhu,Xiangdong Fang

Nature communications 15:1637 PubMed38388640

2024

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Geng,Shuangxi Li,Yu Li,Zhihao Wu,Sunil Bhurtel,Suman Rimal,Danish Khan,Rani Ohja,Onn Brandman,Bingwei Lu

Neural regeneration research 19:671-679 PubMed37721300

2023

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyun Liu,Shangde Guo,Rong Liu,Minfang Guo,Qing Wang,Zhi Chai,Baoguo Xiao,Cungen Ma

Cell death discovery 9:194 PubMed37369660

2023

SOX1 acts as a tumor hypnotist rendering nasopharyngeal carcinoma cells refractory to chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Xing Lei,Shu-Lan Wang,Ying Xia,Min Yan,Bin He,Bo Wang,Zi-Jie Long,Quentin Liu

eLife 11: PubMed36458816

2022

VPS9D1-AS1 overexpression amplifies intratumoral TGF-β signaling and promotes tumor cell escape from CD8 T cell killing in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Yang,Xichen Dong,Zheng Liu,Jinjing Tan,Xiaoxi Huang,Tao Wen,Hao Qu,Zhenjun Wang

Nature communications 12:3492 PubMed34108460

2021

Translation and codon usage regulate Argonaute slicer activity to trigger small RNA biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Meetali Singh,Eric Cornes,Blaise Li,Piergiuseppe Quarato,Loan Bourdon,Florent Dingli,Damarys Loew,Simone Proccacia,Germano Cecere

Molecular cell 81:1425-1438.e10 PubMed33662272

2021

PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqian Shen,Zi Chao Zhang,Shanshan Cheng,An Liu,Jian Zuo,Shuting Xia,Xian Liu,Wenhua Liu,Zhengping Jia,Wei Xie,Junhai Han

Autophagy 17:3592-3606 PubMed33629929

2021

Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.

Applications

Unspecified application

Species

Unspecified reactive species

Tengfei Huang,Tianqi Xu,Yangfan Wang,Yan Zhou,Dandan Yu,Zhiyuan Wang,Linfang He,Zhangpeng Chen,Yaliang Zhang,Don Davidson,Yuyuan Dai,Chunhua Hang,Xiangyu Liu,Chao Yan

View all publications

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重组Anti-RPS3抗体[EPR7808] - Ribosome Marker