Anti-RPS19抗体(ab40833)
Key features and details
- Goat polyclonal to RPS19
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RPS19抗体
参阅全部 RPS19 一抗 -
描述
山羊多克隆抗体to RPS19 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Cat, Dog, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide: with C-
KMVEKDQDGGRK
sequence from the internal region of Human RPS19. -
阳性对照
- WB: K562 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40833于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
1 hour primary incubation is recommended for this product. |
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Required for pre-rRNA processing and maturation of 40S ribosomal subunits. -
组织特异性
Higher level expression is seen in the colon carcinoma tissue than normal colon tissue. -
疾病相关
Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. -
序列相似性
Belongs to the ribosomal protein S19e family. -
细胞定位
Nucleus. Located more specifically in the nucleoli. - Information by UniProt
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数据库链接
- Entrez Gene: 6223 Human
- Entrez Gene: 100503302 Mouse
- Entrez Gene: 20085 Mouse
- Entrez Gene: 29287 Rat
- Omim: 603474 Human
- SwissProt: Q32PD5 Cow
- SwissProt: P39019 Human
- SwissProt: Q9CZX8 Mouse
see all -
别名
- 40S ribosomal protein S19 antibody
- DBA antibody
- DBA1 antibody
see all
图片
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Anti-RPS19 antibody (ab40833) at 0.1 µg/ml + K562 cell lysate in RIPA buffer at 35 µg
Predicted band size: 16 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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Datasheet download
文献 (6)
ab40833 被引用在 6 文献中.
- Khameneh HJ et al. Myc controls NK cell development, IL-15-driven expansion, and translational machinery. Life Sci Alliance 6:N/A (2023). PubMed: 37105715
- Wilkes MC et al. Downregulation of SATB1 by miRNAs reduces megakaryocyte/erythroid progenitor expansion in preclinical models of Diamond-Blackfan anemia. Exp Hematol 111:66-78 (2022). PubMed: 35460833
- Bibikova E et al. TNF-mediated inflammation represses GATA1 and activates p38 MAP kinase in RPS19-deficient hematopoietic progenitors. Blood 124:3791-8 (2014). PubMed: 25270909
- Blalock WL et al. Identification of the PKR nuclear interactome reveals roles in ribosome biogenesis, mRNA processing and cell division. J Cell Physiol 229:1047-60 (2014). PubMed: 24347309
- Horos R et al. Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts. Blood 119:262-72 (2012). WB ; Mouse . PubMed: 22058113
- Ponpuak M et al. Delivery of cytosolic components by autophagic adaptor protein p62 endows autophagosomes with unique antimicrobial properties. Immunity 32:329-41 (2010). ICC/IF ; Mouse . PubMed: 20206555