重组Anti-RPS16抗体[EPR11755] (ab177951)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11755] to RPS16
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RPS16抗体[EPR11755]
参阅全部 RPS16 一抗 -
描述
兔单克隆抗体[EPR11755] to RPS16 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IP, IHC-P, WBmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- MCF-7, fetal liver, HeLa, Ramos, PC-12 and NIH/3T3 cell lysates; Breast carcinoma tissue; MCF-7 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11755 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177951于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
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说明 |
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ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Predicted molecular weight: 16 kDa. |
靶标
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相关性
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS16 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S9P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. -
数据库链接
- Entrez Gene: 6217 Human
- Entrez Gene: 20055 Mouse
- Entrez Gene: 140655 Rat
- Omim: 603675 Human
- SwissProt: P62249 Human
- SwissProt: P14131 Mouse
- SwissProt: P62250 Rat
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别名
- 40S ribosomal protein S16 antibody
- Ribosomal protein S16 antibody
- RPS 16 antibody
- S16 antibody
图片
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Western blot analysis on immunoprecipitation pellet from fetal liver lysate using ab177951.
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Immunofluorescent analysis of MCF-7 cells labeling RPS16 with ab177951 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] (ab177951)
Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labeling RPS16 with ab177951 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-RPS16 antibody [EPR11755] (ab177951) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : Fetal liver lysate
Lane 3 : HeLa cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : PC-12 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 16 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab177951 被引用在 3 文献中.
- Ren J et al. Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin. J Pineal Res 75:e12897 (2023). PubMed: 37391878
- Nakamura S et al. Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement. Int J Mol Sci 24:N/A (2022). PubMed: 36614058
- Wu W et al. MicroRNA let-7 Suppresses Influenza A Virus Infection by Targeting RPS16 and Enhancing Type I Interferon Response. Front Cell Infect Microbiol 12:904775 (2022). PubMed: 35873150