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重组RabMAb

重组Anti-RPS12抗体[EPR10963] (ab175219)

Submit a review Submit a question References (3)
Western blot - Anti-RPS12 antibody [EPR10963] (ab175219)
  • Immunoprecipitation - Anti-RPS12 antibody [EPR10963] (ab175219)
  • Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] (ab175219)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] (ab175219)
  • Anti-RPS12 antibody [EPR10963] (ab175219)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10963] to RPS12
  • Suitable for: WB, IP, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-RPS12抗体[EPR10963]
    参阅全部 RPS12 一抗

  • 描述

    兔单克隆抗体[EPR10963] to RPS12

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, ICC/IF, Flow Cyt (Intra)more details
    不适用于: IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HeLa, MCF-7, HepG2, Raji, C6, Raw264.7 and NIH-3T3 cell lysates; MCF7 cells; Hela cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR10963

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab175219于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/5000. Predicted molecular weight: 15 kDa.
IP
1/10 - 1/100.
ICC/IF
1/50 - 1/100.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
说明
WB
1/1000 - 1/5000. Predicted molecular weight: 15 kDa.
IP
1/10 - 1/100.
ICC/IF
1/50 - 1/100.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
应用说明
Is unsuitable for IHC-P.

靶标

图片

  • Western blot - Anti-RPS12 antibody [EPR10963] (ab175219)
    Western blot - Anti-RPS12 antibody [EPR10963] (ab175219)
    All lanes : Anti-RPS12 antibody [EPR10963] (ab175219) at 1/1000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Raji cell lysate
    Lane 5 : C6 cell lysate
    Lane 6 : RAW 264.7 cell lysate
    Lane 7 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa

  • Immunoprecipitation - Anti-RPS12 antibody [EPR10963] (ab175219)
    Immunoprecipitation - Anti-RPS12 antibody [EPR10963] (ab175219)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175219 at 1/10 dilution.

  • Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] (ab175219)
    Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] (ab175219)

    Intracellular flow cytometric analysis of permeabilized HeLa cells labeling RPS12 with ab175219 at 1/10 dilution (red) compared toa rabbit IgG negative control (green). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] (ab175219)
    Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] (ab175219)

    Immunofluorescent staining of MCF7 cells labeling RPS12 with ab175219 at 1/50 dilution (green). DAPI nuclear staining (blue).

  • Anti-RPS12 antibody [EPR10963] (ab175219)
    Anti-RPS12 antibody [EPR10963] (ab175219)

文献 (3)

发表研究结果有使用 ab175219？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab175219 被引用在 3 文献中.

  • Yang F  et al. Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma. Front Oncol 11:731896 (2021). PubMed: 34485161
  • Brumwell A  et al. Hypoxia influences polysome distribution of human ribosomal protein S12 and alternative splicing of ribosomal protein mRNAs. RNA 26:361-371 (2020). PubMed: 31911497
  • Luo Q  et al. mTORC1 Negatively Regulates the Replication of Classical Swine Fever Virus Through Autophagy and IRES-Dependent Translation. iScience 3:87-101 (2018). PubMed: 30428332

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