Anti-RPP25抗体(ab126338)
Key features and details
- Rabbit polyclonal to RPP25
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RPP25抗体
参阅全部 RPP25 一抗 -
描述
兔多克隆抗体to RPP25 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: ASLSVLKNVP GLAILLSKDA LDPRQPGYQP PNPHPGPSSP PAAPASKRSL GEPAAGEGSA KRSQPEP, corresponding to Human RPP25.
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阳性对照
- WB: SK-MEL-30 whole cell lysates; IHC-P: Human colon, kidney and testis tissue; ICC: SiHa whole cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126338于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa.
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| 说明 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa. |
靶标
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功能
Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. This subunit binds to RNA. -
序列相似性
Belongs to the histone-like Alba family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 54913 Human
- SwissProt: Q9BUL9 Human
- Unigene: 8562 Human
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别名
- FLJ20374 antibody
- Ribonuclease P protein subunit p25 antibody
- RNase P protein subunit p25 antibody
see all
图片
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Western blot - Anti-RPP25 antibody (ab126338)Anti-RPP25 antibody (ab126338) at 0.4 µg/ml + SK-MEL-30 (Human cutaneous melanoma cell line) whole cell lysate
Predicted band size: 21 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)Immunohistochemical analysis of human colon tissue labeling RPR25 in the nucleus of glandular cells with ab126338 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-RPP25 antibody (ab126338)Immunocytochemical analysis of SiHa (Human cervical carcinoma cell line) whole cells labeling RPR25 in the nucleoplasm and microtubule organizing center with ab126338 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)Immunohistochemical analysis of human kidney tissue labeling RPR25 in the nucleus of cells in tubules with ab126338 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)Immunohistochemical analysis of human testis tissue labeling RPR25 in the nucleus of Leydig cells with ab126338 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)Immunohistochemical analysis of human skeletal muscle tissue labeling RPR25 with ab126338 at a 1/50 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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